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experimentelisa
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<span>Documentation</span>
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<main class="main">
</ul></nav><h1 class="main-title">ExperimentELISA</h1><div class="iconography" data-src="s= d= m= c= u= l= n= e="></div><div class="functionIntroWrap">
<div class="functionIntro">
<p class="code">
<span class="IF"><a class="package-symbol" href="/SLL/Experiment/ref/ExperimentELISA.html">ExperimentELISA</a><span class="openbracket">[</span><span class="TI">Samples</span><span class="closebracket">]</span><span class="special-character DoubleLongRightArrow">⟹</span><span class="TI">Protocol</span></span>
</p>
<p class="code-description">creates a <span class="TI">Protocol</span> to run Enzyme-Linked Immunosorbent Assay (ELISA) experiment on the provided <span class="TI">Samples</span> for the detection and quantification of certain analytes.</p>
</div>
</div> <div class="ExperimentDescription" id="1970391283"><a name="1970391283"></a>Enzyme-Linked Immunosorbent Assay (ELISA) uses antigen-antibody interaction to quantitatively measure the concentration of an antigen in samples or compare antibody binding affinity. There are many forms of ELISA, such as direct ELISA, indirect ELISA, sandwich ELISA, competitive ELISA, and fast ELISA that apply antigens and antibodies in varied sequences. During an ELISA experiment, protein standards and samples are loaded onto ELISA plates and the target antigen is bound by antibodies. Antigen concentration is revealed by an enzyme, which converts its substrate into a colored product. Results are measured using colorimetric detection, and antigen concentrations are calculated using the standard curve generated from protein standards.</div> <section id="DetailsAndOptions"><div class="inner">
<h1 class="toggle open">Experimental Principles</h1>
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<ul class="functionList">
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<div class="Figure" id="1908607227"><a name="1908607227"></a><strong>Figure 1.1: </strong>Overview of ELISA methods. In a DirectELISA experiment, the Sample is coated on the ELISAPlate, and then a primary antibody is used to detect the target antigen. In a DirectSandwichELISA experiment, a capture antibody is coated onto the ELISAPlate to pull down the target antigen from the sample. Then primary antibody is used to detect the target antigen. In a DirectCompetitiveELISA experiment, a reference antigen is used to coat the ELISAPlate. Samples are incubated with the primary antibody. Then, when the Sample-Antibody-Complex solution is loaded on the ELISAPlate, the remaining free primary antibody binds to the reference antigen. In a FastELISA experiment, a coating antibody against a tag is coated to the ELISAPlate. A capture antibody containing this small tag and a primary antibody for antigen detection is incubated with the sample to form a CaptureAntibody-TargetAntigen-PrimaryAntibody complex. Then this complex is pulled down by the coating antibody to the surface of the plate. Compared with the Direct methods where the primary antibody is conjugated with an enzyme (such as Horse Radish Peroxidase/HRP or Alkaline Phosphatas/AP) for detection, in Indirect methods a secondary antibody is, instead, conjugated with this enzyme. During Indirect methods, an additional step of SecondaryAntibody incubation is added to the corresponding Direct methods.</div>
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<div class="Figure" id="158144992"><a name="158144992"></a><strong>Figure 1.2: </strong>DirectELISA process: 1. If required, antibodies are diluted. 2. Sample, Spike, Standard, Blank and any dilutions are assembled in 96-well 2ml deep well plate. 3. Assembled samples from the previous step are transferred to the ELISA plate and incubated to coat the plate. 4. Blocking buffer is added to the ELISA plate to prevent non-specific binding. 5. Primary antibody is added to the plate for immunosorbence. 5. SubstrateSolution and StopSolution (if required) are added to the plate for colorimetric detection.</div>
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<div class="Figure" id="974095834"><a name="974095834"></a><strong>Figure 1.3: </strong>IndirectELISA process: 1. If required, antibodies are diluted. 2. Sample, Spike, Standard, Blank and any dilutions are assembled in 96-well 2ml deep well plate. 3. Assembled samples from the previous step are transferred to the ELISA plate and incubated to coat the plate. 4. Blocking buffer is added to the ELISA plate to prevent non-specific binding. 5. Primary antibody and secondary antibody are sequentially added to the plate for immunosorbence. 5. SubstrateSolution and StopSolution (if required) are added to the plate for colorimetric detection.</div>
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<div class="Figure" id="953123749"><a name="953123749"></a><strong>Figure 1.4: </strong>DirectSandwichELISA process: 1. If required, antibodies are diluted. 2. Capture antibody is added to the ELISA plate for coating. 3. Sample, Spike, Standard, Blank and any dilutions are assembled in 96-well 2ml deep well plate. 4. Blocking buffer is added to the ELISA plate to prevent non-specific binding. 5. Assembled samples and primary antibody are added to the plate sequentially for immunosorbence. 6. SubstrateSolution and StopSolution (if required) are added to the plate for colorimetric detection.</div>
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<div class="Figure" id="1653954018"><a name="1653954018"></a><strong>Figure 1.5: </strong>IndirectSandwichELISA process: 1. If required, antibodies are diluted. 2. Capture antibody is added to the ELISA plate for coating. 3. Sample, Spike, Standard, Blank and any dilutions are assembled in 96-well 2ml deep well plate. 4. Blocking buffer is added to the ELISA plate to prevent non-specific binding. 5. Assembled samples, primary antibody, and secondary antibody are added to the plate sequentially for immunosorbence. 6. SubstrateSolution and StopSolution (if required) are added to the plate for colorimetric detection.</div>
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<div class="Figure" id="468629391"><a name="468629391"></a><strong>Figure 1.6: </strong>DirectCompetitiveELISA process: 1. If required, antibodies and reference antigen are diluted. 2. Reference antigen is added to the ELISA plate for coating. 3. Sample, Spike, Standard, Blank and any dilutions are assembled in 96-well 2ml deep well plate and primary antibody is mixed with each sample and incubated (if required). 4. Blocking buffer is added to the ELISA plate to prevent non-specific binding. 5. The Sample-antibody mixture from step 3 is added to the ELISA plate for immunosorbence. 6. SubstrateSolution and StopSolution (if required) are added to the plate for colorimetric detection.</div>
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<div class="Figure" id="1183304328"><a name="1183304328"></a><strong>Figure 1.7: </strong>IndirectCompetitiveELISA process: 1. If required, antibodies and reference antigen are diluted. 2. Reference antigen is added to the ELISA plate for coating. 3. Sample, Spike, Standard, Blank and any dilutions are assembled in 96-well 2ml deep well plate and primary antibody is mixed with each sample and incubated (if required). 4. Blocking buffer is added to the ELISA plate to prevent non-specific binding. 5. The Sample-antibody mixture from step 3 is added to the ELISA plate, and then the secondary antibody is added, for immunosorbence. 6. SubstrateSolution and StopSolution (if required) are added to the plate for colorimetric detection.</div>
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<div class="Figure" id="11501274"><a name="11501274"></a><strong>Figure 1.8: </strong>FastELISA process: 1. If required, antibodies are diluted. 2. Coating antibody is added to the ELISA plate for coating. 3. Sample, Spike, Standard, Blank and any dilutions are assembled in 96-well 2ml deep well plate. Then primary antibody and capture antibody are mixed with each sample and incubated (if required). 4. Blocking buffer is added to the ELISA plate to prevent non-specific binding. 5. The Sample-antibody mixture from step 3 is added to the ELISA plate for immunosorbence. 6. SubstrateSolution and StopSolution (if required) are added to the plate for colorimetric detection.</div>
</ul>
</div>
</div></section>
<section id="DetailsAndOptions"><div class="inner">
<h1 class="toggle open">Instrumentation</h1>
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<h3 class="Subsubsection"><a name="1798276472"></a>Hamilton Nimbus HD</h3>
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<div class="Figure" id="45757825"><a name="45757825"></a><strong>Figure 2.1.1: </strong>An overview of the ELISA robotic workcell. It is capable of processing plate operations including liquid handling with 4 indipendent pipettes, microplate movements, plate incubation-shaking, plate washing, and plate absorbance measurements.</div>
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<section id="DetailsAndOptions"><div class="inner">
<h1 class="toggle">Experiment Options</h1>
<div class="hideable">
<ul class="functionList">
<h3 class="Subsubsection"><a name="219191393"></a>General</h3>
<h4 class="Subsubsubsection"><a name="1015287546"></a><strong>Method</strong></h4>
<div class="Text"><a name="32726560"></a>Defines the type of ELISA experiment to be performed. Types include DirectELISA, IndirectELISA, DirectSandwichELISA, IndirectSandwichELISA, DirectCompetitiveELISA, IndirectCompetitiveELISA, and FastELISA. Compared with the Direct methods where the primary antibody is conjugated with an enzyme (such as Horse Radish Peroxidase/HRP or Alkaline Phosphatas/AP) for detection, in all Indirect methods a secondary antibody is, instead, conjugated with this enzyme. In Indirect methods, an additional step of SecondaryAntibody incubation is added to the corresponding Direct methods. In a DirectELISA experiment, the Sample is coated on the ELISAPlate, and then a primary antibody is used to detect the target antigen. In a DirectSandwichELISA experiment, a capture antibody is coated onto the ELISAPlate to pull down the target antigen from the sample. Then a primary antibody is used to detect the target antigen. In a DirectCompetitiveELISA experiment, a reference antigen is used to coat the ELISAPlate. Samples are incubated with the primary antibody. Then, when the Sample-Antibody-Complex solution is loaded on the ELISAPlate, the remaining free primary antibody binds to the reference antigen. In a FastELISA experiment, a coating antibody against a tag is coated to the ELISAPlate. A capture antibody containing this tag and a primary antibody for antigen detection is incubated with the sample to form a CaptureAntibody-TargetAntigen-PrimaryAntibody complex. Then this complex is pulled down by the coating antibody to the surface of the plate (See Figure 1.1).</div>
<div class="Bullet" id="279480288"><a name="279480288"></a>Default Value: DirectELISA</div>
<div class="Bullet" id="678464427"><a name="678464427"></a>Pattern Description: DirectELISA, IndirectELISA, DirectSandwichELISA, IndirectSandwichELISA, DirectCompetitiveELISA, IndirectCompetitiveELISA, or FastELISA.</div>
<div class="Bullet" id="1960942666"><a name="1960942666"></a>Programmatic Pattern: ELISAMethodP</div>
<h4 class="Subsubsubsection"><a name="131856953"></a><strong>TargetAntigen</strong></h4>
<div class="Text"><a name="2064169014"></a>The Analyte molecule (e.g., peptide, protein, and hormone) detected and quantified in the samples by Antibodies in the ELISA experiment. This option is used to automatically set sample Antibodies and the corresponding experiment conditions of Standards and Blanks.</div>
<div class="Bullet" id="2037784660"><a name="2037784660"></a>Default Value: Automatic</div>
<div class="Bullet" id="977578264"><a name="977578264"></a>Default Calculation: Automatically set to the Model[Molecule] in the Analyte field of the sample.</div>
<div class="Bullet" id="1907983498"><a name="1907983498"></a>Pattern Description: An object of type or subtype Model[Molecule] or Null.</div>
<div class="Bullet" id="1252833882"><a name="1252833882"></a>Programmatic Pattern: (ObjectP[{Model[Molecule]}] | Automatic) | Null</div>
<div class="Bullet" id="301895939"><a name="301895939"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1608672559"></a><strong>NumberOfReplicates</strong></h4>
<div class="Text"><a name="140583383"></a>The number of times an ELISA assay will be repeated in parallel wells. Samples, Standards, or Blanks will be loaded this number of times in the plates. Replications are conducted at the same time, and if possible, in the same ELISAPlate. If set to Null, each Sample, Standard, or Blank will be assayed once.</div>
<div class="Bullet" id="552906381"><a name="552906381"></a>Default Value: Automatic</div>
<div class="Bullet" id="535804898"><a name="535804898"></a>Default Calculation: Automatically set to 2 unless method is DirectELISA or IndirectELISA and Coating is False, in which case it is set to Null.</div>
<div class="Bullet" id="873988815"><a name="873988815"></a>Pattern Description: Greater than or equal to 2 and less than or equal to 192 in increments of 1 or Null.</div>
<div class="Bullet" id="341868225"><a name="341868225"></a>Programmatic Pattern: (RangeP[2, 192, 1] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1260501850"></a><strong>WashingBuffer</strong></h4>
<div class="Text"><a name="328415295"></a>The solution used to rinse off unbound molecules from the assay plate.</div>
<div class="Bullet" id="642890275"><a name="642890275"></a>Default Value: Model[Sample, StockSolution, Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4]</div>
<div class="Bullet" id="1066623868"><a name="1066623868"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample.</div>
<div class="Bullet" id="1701347967"><a name="1701347967"></a>Programmatic Pattern: ObjectP[{Model[Sample], Object[Sample]}] | _String</div>
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<h3 class="Subsubsection"><a name="947153227"></a>Sample Assembly</h3>
<h4 class="Subsubsubsection"><a name="1655494625"></a><strong>Spike</strong></h4>
<div class="Text"><a name="1100031843"></a>The sample with a known concentration of analyte. Spike is to be mixed with the input sample. The purpose of spiking is to perform a spike-and-recovery assessment to determine whether the ELISA can accurately test the concentration of analyte within the context of the sample. If the recovery observed for the spiked sample is identical to the analyte prepared in standard diluent, the sample is considered not to interfere with the assay. For example, if molecules in the sample inhibits the binding between the antibody and TargetAntigen, the results from the ELISA becomes inaccurate. In a Spike-and-Recovery experiment,an aliquot of a sample is mixed with Spike. ELISA is performed on the Sample alone (also called Neat Sample) and the Spiked Sample at the same time, where the Spike concentration in the sample can be measured. This measured Spike concentration can be then compared with the known Spike concentration. Typically a 20% difference between measured Spike concentration and the known Spike concentration is acceptable. Spiked sample can be further diluted to perform linearity-of-dilution assessment. The plate used for sample spiking and dilution will be discarded after the experiment.</div>
<div class="Bullet" id="548468278"><a name="548468278"></a>Default Value: Null</div>
<div class="Bullet" id="1106535422"><a name="1106535422"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="332454534"><a name="332454534"></a>Programmatic Pattern: (ObjectP[{Model[Sample], Object[Sample]}] | _String) | Null</div>
<div class="Bullet" id="1958387091"><a name="1958387091"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="129980868"></a><strong>SpikeDilutionFactor</strong></h4>
<div class="Text"><a name="597579477"></a>The dilution ratio by which the Spike is mixed with the input sample before further dilution is performed.</div>
<div class="Bullet" id="1884736567"><a name="1884736567"></a>Default Value: Automatic</div>
<div class="Bullet" id="1383688319"><a name="1383688319"></a>Default Calculation: Automatically set to 0.1 if Spike is not Null.</div>
<div class="Bullet" id="549164070"><a name="549164070"></a>Pattern Description: Greater than or equal to 0 and less than or equal to 1 or Null.</div>
<div class="Bullet" id="2063293331"><a name="2063293331"></a>Programmatic Pattern: (RangeP[0, 1] | Automatic) | Null</div>
<div class="Bullet" id="205082361"><a name="205082361"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="107987892"></a><strong>SpikeStorageCondition</strong></h4>
<div class="Text"><a name="328819129"></a>The condition under which the unused portion of spike should be stored.</div>
<div class="Bullet" id="987677372"><a name="987677372"></a>Default Value: Automatic</div>
<div class="Bullet" id="1992483541"><a name="1992483541"></a>Default Calculation: Automatically set to Refrigerator if Spike is not Null.</div>
<div class="Bullet" id="1958365916"><a name="1958365916"></a>Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, BacteriaIncubation, MammalianIncubation, TissueCultureCellsIncubation, MicrobialCellsIncubation, MicrobialCellsShakingIncubation, YeastCellsIncubation, YeastCellsShakingIncubation, ViralIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.</div>
<div class="Bullet" id="1921576290"><a name="1921576290"></a>Programmatic Pattern: ((SampleStorageTypeP | Disposal) | Automatic) | Null</div>
<div class="Bullet" id="2073622444"><a name="2073622444"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="2007792792"></a><strong>SampleSerialDilutionCurve</strong></h4>
<div class="Text"><a name="1562909833"></a>The collection of serial dilutions that will be performed on each sample: if spiking was performed, the dilution will be performed on the spiked sample; otherwise, the dilutions will be performed on the sample. This Linearity-of-Dilution step assesses whether the ELISA experiment can reliably measure TargetAntigen concentration at different concentration ranges. For example, a sample with TargetAntigen concentration of 100ng/ul, if diluted 1:2, 1:4, and 1:8, should yield ELISA measurements of 50ng/ul, 25ng/ul, and 12.5ng/ul (or should follow a concentration ratio of 4:2:1). Typically a 20% difference between the measured and expected concentrations is considered acceptable. For Serial Dilution Volumes, the Transfer Volume is taken out of the sample and added to a second well with the Diluent Volume of the Diluent. It is mixed, then the Transfer Volume is taken out of that well to be added to a third well. This is repeated to make Number Of Dilutions diluted samples. For example, if a 100 ug/ ml sample with a Transfer Volume of 20 Microliters, a Diluent Volume of 60 Microliters and a Number of Dilutions of 3 is used, it will create a DilutionCurve of 25 ug/ ml, 6.25 ug/ ml, and 1.5625 ug/ ml with each dilution having a volume of 60 Microliters. For Serial Dilution Factors, the sample will be diluted by the dilution factor at each transfer step. IMPORTANT: because the dilution curve does not intrinsically include the original sample, in the case of sample dilution the first diluting factor should be 1 or Diluent Volume should be 0 Microliter to include the original sample. For example, if a Spike-and-Recovery assay is to be performed, the first dilution factor must be 1 in order to include the original spiked sample. During experiment, an 5% extra volume than specified is going to be prepared in order to offset pipetting errors. Therefore, the total volume for each well specified in this option should be equal or greater than the volume needed for the experiment. Because the dilution of each assay (well) is prepared independently, the Number of Replications should not be considered when determining the Spike Volume. The plate used for sample dilutions will be discarded after the experiment. Note: if spike or antibodies are to be mixed with samples, their volumes are counted towards diluent volume.</div>
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<div class="Figure" id="1073796223"><a name="1073796223"></a><strong>Figure 3.1: </strong>Use the SerialDilutionCuve option to create a collection of serial dilutions that will be performed on samples.</div>
<div class="Bullet" id="1988034220"><a name="1988034220"></a>Default Value: Automatic</div>
<div class="Bullet" id="193071867"><a name="193071867"></a>Default Calculation: The option is automatically set Null if or a SampleDilutionCurve is specified.</div>
<div class="Bullet" id="111078052"><a name="111078052"></a>Pattern Description: Serial Dilution Factor or Serial Dilution Volumes or Null.</div>
<div class="Bullet" id="725530721"><a name="725530721"></a>Programmatic Pattern: (({RangeP[10*Microliter, 1500*Milliliter], {RangeP[0, 1], GreaterP[0, 1]} | {RangeP[0, 1]..}} | {RangeP[0*Microliter, 1000*Microliter], RangeP[0*Microliter, 1000*Microliter], GreaterP[0, 1]}) | Automatic) | Null</div>
<div class="Bullet" id="582680181"><a name="582680181"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="580371747"></a><strong>SampleDilutionCurve</strong></h4>
<div class="Text"><a name="437227155"></a>The collection of dilutions that will be performed on each sample: the dilutions will be performed on the sample. Spiked samples should only be further diluted with serial dilution. This Linearity-of-Dilution step assesses whether the ELISA experiment can reliably measure TargetAntigen concentration at different concentration ranges. For example, a sample with TargetAntigen concentration of 100ng/ul, if diluted 1:2, 1:4, and 1:8, should yield ELISA measurements of 50ng/ul, 25ng/ul, and 12.5ng/ul (or should follow a concentration ratio of 8:4:2:1). Typically a 20% difference between the measured and expected concentrations is considered acceptable. Linearity-of-Dilution experiment can be done with Spiked or non-Spiked samples. For Fixed Dilution Volume Dilution Curves, the Spike Amount is the volume of the sample that will be mixed the Diluent Volume of the Diluent to create a desired concentration. The Assay Volume is the TOTAL volume of the sample that will be created after being diluted by the Dilution Factor. IMPORTANT: because the dilution curve does not intrinsically include the original sample, in the case of sample dilution the first diluting factor must be 1, or Diluent Volume should be 0 to include the original sample. During experiment, an 5% extra volume than specified is going to be prepared in order to offset pipetting errors. Therefore, the total volume for each well specified in this option should be equal or greater than the volume needed for the experiment. Because the dilution of each assay (well) is prepared independently, the Number of Replications should not be considered when determining the Spike Volume. The plate used for sample dilutions will be discarded after the experiment. Note: if spike or antibodies are to be mixed with samples, their volumes are counted towards diluent volume.</div>
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<div class="Figure" id="1900156471"><a name="1900156471"></a><strong>Figure 3.2: </strong>Use the DilutionCuve option to create a collection of dilutions that will be performed on samples.</div>
<div class="Bullet" id="700493560"><a name="700493560"></a>Default Value: Automatic</div>
<div class="Bullet" id="689910066"><a name="689910066"></a>Default Calculation: The option is automatically set Null if or a SampleSerialDilutionCurve is specified.</div>
<div class="Bullet" id="1054269109"><a name="1054269109"></a>Pattern Description: Fixed Dilution Factor or Fixed Dilution Volume or Null.</div>
<div class="Bullet" id="213343566"><a name="213343566"></a>Programmatic Pattern: (({{RangeP[10*Microliter, 1500*Microliter], RangeP[0, 1]}..} | {{RangeP[0*Microliter, 1000*Microliter], RangeP[0*Microliter, 1000*Microliter]}..}) | Automatic) | Null</div>
<div class="Bullet" id="1515106919"><a name="1515106919"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1688385154"></a><strong>SampleDiluent</strong></h4>
<div class="Text"><a name="1987855467"></a>The buffer used to perform multiple dilutions of samples or spiked samples.</div>
<div class="Bullet" id="1483737490"><a name="1483737490"></a>Default Value: Automatic</div>
<div class="Bullet" id="595885466"><a name="595885466"></a>Default Calculation: If SampleDilutionCurve and SampleSerialDilutionCurve are not both Null, in the case when Method is set to DirectELISA and IndirectELISA, the option is automatically set to Model[Sample, StockSolution, "1x Carbonate-Bicarbonate Buffer pH10"]; in all other cases, the option is automatically set to Model[Sample,"ELISA Blocker Blocking Buffer"].</div>
<div class="Bullet" id="1330781101"><a name="1330781101"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="395781002"><a name="395781002"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
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<h3 class="Subsubsection"><a name="1271666919"></a>Antibody Antigen Preparation</h3>
<h4 class="Subsubsubsection"><a name="1148652689"></a><strong>CoatingAntibody</strong></h4>
<div class="Text"><a name="1675241140"></a>The sample containing the antibody that is used for coating in FastELISA.</div>
<div class="Bullet" id="119744163"><a name="119744163"></a>Default Value: Automatic</div>
<div class="Bullet" id="347970302"><a name="347970302"></a>Default Calculation: If Method is FastELISA, automatically set to an antibody against a tag which is conjugated to a the CaptureAntibody.</div>
<div class="Bullet" id="738894933"><a name="738894933"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="1856691977"><a name="1856691977"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<div class="Bullet" id="337182882"><a name="337182882"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1970836988"></a><strong>CoatingAntibodyDilutionFactor</strong></h4>
<div class="Text"><a name="1069485988"></a>The dilution ratio of CoatingAntibody. CoatingAntibody is diluted with CoatingAntibodyDiluent. Either CoatingAntibodyDilutionFactor or CoatingAntibodyVolume should be provided but not both.</div>
<div class="Bullet" id="412874926"><a name="412874926"></a>Default Value: Automatic</div>
<div class="Bullet" id="669217825"><a name="669217825"></a>Default Calculation: Automatically set to 0.001 (1:1,000) for FastELISA.</div>
<div class="Bullet" id="1172125436"><a name="1172125436"></a>Pattern Description: Greater than or equal to 0 and less than or equal to 1 or Null.</div>
<div class="Bullet" id="1044708921"><a name="1044708921"></a>Programmatic Pattern: (RangeP[0, 1] | Automatic) | Null</div>
<div class="Bullet" id="596537375"><a name="596537375"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1549997429"></a><strong>CoatingAntibodyVolume</strong></h4>
<div class="Text"><a name="1830151093"></a>The volume of undiluted CoatingAntibody added into the corresponding well of the assay plate. CoatingAntibody is diluted with CoatingAntibodyDiluent. CoatingAntibodyVolume is used as an alternative to CoatingAntibodyDilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.</div>
<div class="Bullet" id="941144070"><a name="941144070"></a>Default Value: Null</div>
<div class="Bullet" id="1591324335"><a name="1591324335"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="626758607"><a name="626758607"></a>Programmatic Pattern: RangeP[0*Microliter, 200*Microliter] | Null</div>
<div class="Bullet" id="524619314"><a name="524619314"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1856439463"></a><strong>CoatingAntibodyDiluent</strong></h4>
<div class="Text"><a name="388147378"></a>The buffer used to dilute CoatingAntibodies.</div>
<div class="Bullet" id="1685146107"><a name="1685146107"></a>Default Value: Automatic</div>
<div class="Bullet" id="1236283713"><a name="1236283713"></a>Default Calculation: If Method is set to FastELISA, the option is automatically set to Model[Sample, StockSolution, "1x Carbonate-Bicarbonate Buffer pH10"].</div>
<div class="Bullet" id="1316079666"><a name="1316079666"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="269382362"><a name="269382362"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="613830258"></a><strong>CoatingAntibodyStorageCondition</strong></h4>
<div class="Text"><a name="133706018"></a>The condition under which the unused portion of Coating Antibody stock sample should be stored.</div>
<div class="Bullet" id="929149764"><a name="929149764"></a>Default Value: Automatic</div>
<div class="Bullet" id="1866486211"><a name="1866486211"></a>Default Calculation: Automatically set to Refrigerator if Method is set to FastELISA.</div>
<div class="Bullet" id="1740116548"><a name="1740116548"></a>Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, BacteriaIncubation, MammalianIncubation, TissueCultureCellsIncubation, MicrobialCellsIncubation, MicrobialCellsShakingIncubation, YeastCellsIncubation, YeastCellsShakingIncubation, ViralIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.</div>
<div class="Bullet" id="1706517390"><a name="1706517390"></a>Programmatic Pattern: ((SampleStorageTypeP | Disposal) | Automatic) | Null</div>
<div class="Bullet" id="1793668045"><a name="1793668045"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1950879376"></a><strong>CaptureAntibody</strong></h4>
<div class="Text"><a name="649350036"></a>The sample containing the antibody that is used to pull down the antigen from sample solution to the surface of the assay plate wells in DirectSandwichELISA, IndirectSandwichELISA, and FastELISA.</div>
<div class="Bullet" id="114160998"><a name="114160998"></a>Default Value: Automatic</div>
<div class="Bullet" id="1000996615"><a name="1000996615"></a>Default Calculation: If Method is FastELISA, automatically set to an antibody containing an affinity tag and against the TargetAntigen. If Method is DirectSandwichELISA or IndirectSandwichELISA, automatically set to an un-tagged antibody against TargetAntigen.</div>
<div class="Bullet" id="363217707"><a name="363217707"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="1451476775"><a name="1451476775"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<div class="Bullet" id="1703411152"><a name="1703411152"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1126733507"></a><strong>CaptureAntibodyDilutionFactor</strong></h4>
<div class="Text"><a name="499252903"></a>The dilution ratio of CaptureAntibody. For DirectSandwichELISA and IndirectSandwichELISA, CaptureAntibody is diluted with CaptureAntibodyDiluent. For FastELISA, CaptureAntibody is diluted in the corresponding sample. Either CaptureAntibodyDilutionFactor or CaptureAntibodyVolume should be provided but not both.</div>
<div class="Bullet" id="724931892"><a name="724931892"></a>Default Value: Automatic</div>
<div class="Bullet" id="1255579413"><a name="1255579413"></a>Default Calculation: For DirectSandwichELISA and IndirectSandwichELISA, automatically set to 0.001 (1:1,000). For FastELISA, automatically set to 0.01 (1:100).</div>
<div class="Bullet" id="1348878869"><a name="1348878869"></a>Pattern Description: Greater than or equal to 0 and less than or equal to 1 or Null.</div>
<div class="Bullet" id="1769773551"><a name="1769773551"></a>Programmatic Pattern: (RangeP[0, 1] | Automatic) | Null</div>
<div class="Bullet" id="1935560707"><a name="1935560707"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="886517793"></a><strong>CaptureAntibodyVolume</strong></h4>
<div class="Text"><a name="463004065"></a>The volume of undiluted CaptureAntibody added into the corresponding well of the assay plate. CaptureAntibodyVolume is used as an alternative to CaptureAntibodyDilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.</div>
<div class="Bullet" id="1376067374"><a name="1376067374"></a>Default Value: Null</div>
<div class="Bullet" id="1312960275"><a name="1312960275"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="1528869000"><a name="1528869000"></a>Programmatic Pattern: RangeP[0*Microliter, 200*Microliter] | Null</div>
<div class="Bullet" id="1059446645"><a name="1059446645"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1332845238"></a><strong>CaptureAntibodyDiluent</strong></h4>
<div class="Text"><a name="727993209"></a>The buffer used to dilute CaptureAntibodies. Set to Null when CapturaAntibody should be diluted into samples (in FastELISA).</div>
<div class="Bullet" id="1167526704"><a name="1167526704"></a>Default Value: Automatic</div>
<div class="Bullet" id="1063231489"><a name="1063231489"></a>Default Calculation: If Method is set to DirectSandwichELISA or IndirectSandwichELISA, the option is automatically set to Model[Sample, StockSolution, "1x Carbonate-Bicarbonate Buffer pH10"].</div>
<div class="Bullet" id="520208936"><a name="520208936"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="745660415"><a name="745660415"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1747514660"></a><strong>CaptureAntibodyStorageCondition</strong></h4>
<div class="Text"><a name="1492534248"></a>The condition under which the unused portion of Capture Antibody stock sample should be stored.</div>
<div class="Bullet" id="266625529"><a name="266625529"></a>Default Value: Automatic</div>
<div class="Bullet" id="1524378261"><a name="1524378261"></a>Default Calculation: If Method is set to DirectSandwichELISA, IndirectSandwichELISA, or FastELISA, Automatically set to Refrigerator.</div>
<div class="Bullet" id="745523917"><a name="745523917"></a>Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, BacteriaIncubation, MammalianIncubation, TissueCultureCellsIncubation, MicrobialCellsIncubation, MicrobialCellsShakingIncubation, YeastCellsIncubation, YeastCellsShakingIncubation, ViralIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.</div>
<div class="Bullet" id="1600876421"><a name="1600876421"></a>Programmatic Pattern: ((SampleStorageTypeP | Disposal) | Automatic) | Null</div>
<div class="Bullet" id="107394484"><a name="107394484"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1092829108"></a><strong>ReferenceAntigen</strong></h4>
<div class="Text"><a name="1898669012"></a>The sample containing the antigen that is used in DirectCompetitiveELISA or IndirectCompetitiveELISA. The ReferenceAntigen competes with TargetAntigen in the samples for the binding of the PrimaryAntibody. Reference Antigen is also referred to as Inhibitor Antigen.</div>
<div class="Bullet" id="1470573911"><a name="1470573911"></a>Default Value: Automatic</div>
<div class="Bullet" id="546428854"><a name="546428854"></a>Default Calculation: Automatically set to a sample containing known amount of TargetAntigen when Method is set to DirectCompetitiveELISA or IndirectCompetitiveELISA.</div>
<div class="Bullet" id="1189674606"><a name="1189674606"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="1788349472"><a name="1788349472"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<div class="Bullet" id="608452492"><a name="608452492"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="2091389677"></a><strong>ReferenceAntigenDilutionFactor</strong></h4>
<div class="Text"><a name="2121450890"></a>The dilution ratio of ReferenceAntigen. The ReferenceAntigenSample is always diluted in ReferenceAntigenDiluent. Either ReferenceAntigenDilutionFactor or ReferenceAntigenVolume should be provided but not both.</div>
<div class="Bullet" id="554117089"><a name="554117089"></a>Default Value: Automatic</div>
<div class="Bullet" id="1556821431"><a name="1556821431"></a>Default Calculation: If Method is DirectCompetitiveELISA or IndirectCompetitiveELISA, automatically set to 0.001 (1:1,000).</div>
<div class="Bullet" id="557035769"><a name="557035769"></a>Pattern Description: Greater than or equal to 0 and less than or equal to 1 or Null.</div>
<div class="Bullet" id="1218952310"><a name="1218952310"></a>Programmatic Pattern: (RangeP[0, 1] | Automatic) | Null</div>
<div class="Bullet" id="2084965437"><a name="2084965437"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1523431560"></a><strong>ReferenceAntigenVolume</strong></h4>
<div class="Text"><a name="2014189386"></a>The volume of undiluted ReferenceAntigen added into the corresponding well of the assay plate. ReferenceAntigenVolume is used as an alternative to ReferenceAntigenDilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.</div>
<div class="Bullet" id="1696282841"><a name="1696282841"></a>Default Value: Null</div>
<div class="Bullet" id="1580294762"><a name="1580294762"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="2083802485"><a name="2083802485"></a>Programmatic Pattern: RangeP[0*Microliter, 200*Microliter] | Null</div>
<div class="Bullet" id="1305050119"><a name="1305050119"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1726863222"></a><strong>ReferenceAntigenDiluent</strong></h4>
<div class="Text"><a name="148682949"></a>The buffer used to dilute the ReferenceAntigen.</div>
<div class="Bullet" id="1390842382"><a name="1390842382"></a>Default Value: Automatic</div>
<div class="Bullet" id="528416679"><a name="528416679"></a>Default Calculation: Method is DirectCompetitiveELISA and IndirectCompetitiveELISA, the option is automatically set to Model[Sample, StockSolution, "1x Carbonate-Bicarbonate Buffer pH10"].</div>
<div class="Bullet" id="1254243608"><a name="1254243608"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="372840704"><a name="372840704"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="2123913829"></a><strong>ReferenceAntigenStorageCondition</strong></h4>
<div class="Text"><a name="1146543569"></a>The condition under which the unused portion of Reference Antigen stock sample should be stored.</div>
<div class="Bullet" id="586999652"><a name="586999652"></a>Default Value: Automatic</div>
<div class="Bullet" id="163276846"><a name="163276846"></a>Default Calculation: Automatically set to Refrigerator if Method is DirectCompetitiveELISA and IndirectCompetitiveELISA.</div>
<div class="Bullet" id="1857333503"><a name="1857333503"></a>Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, BacteriaIncubation, MammalianIncubation, TissueCultureCellsIncubation, MicrobialCellsIncubation, MicrobialCellsShakingIncubation, YeastCellsIncubation, YeastCellsShakingIncubation, ViralIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.</div>
<div class="Bullet" id="381892129"><a name="381892129"></a>Programmatic Pattern: ((SampleStorageTypeP | Disposal) | Automatic) | Null</div>
<div class="Bullet" id="1779874867"><a name="1779874867"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="2057170606"></a><strong>PrimaryAntibody</strong></h4>
<div class="Text"><a name="379658342"></a>The antibody that directly binds with the TargetAntigen (analyte).</div>
<div class="Bullet" id="743838757"><a name="743838757"></a>Default Value: Automatic</div>
<div class="Bullet" id="1195679712"><a name="1195679712"></a>Default Calculation: The option will be automatically set to an antibody against the TargetAntigen.</div>
<div class="Bullet" id="1784434605"><a name="1784434605"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="1383275880"><a name="1383275880"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<div class="Bullet" id="59752738"><a name="59752738"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1389404417"></a><strong>PrimaryAntibodyDilutionFactor</strong></h4>
<div class="Text"><a name="2130342688"></a>The dilution ratio of PrimaryAntibody. For DirectELISA, IndirectELISA, DirectSandwichELISA, and IndirectSandwichELISA, the antibody is diluted with PrimaryAntibodyDiluent. For DirectCompetitiveELISA, IndirectCompetitiveELISA, and FastELISA, the antibody is diluted with the corresponding sample. Either PrimaryAntibodyDilutionFactor or PrimaryAntibodyVolume should be provided but not both.</div>
<div class="Bullet" id="1822194432"><a name="1822194432"></a>Default Value: Automatic</div>
<div class="Bullet" id="357528757"><a name="357528757"></a>Default Calculation: If used for DirectELISA, IndirectELISA, DirectSandwichELISA, IndirectSandwichELISA, automatically set to 0.001 (1:1,000). If used for DirectCompetitiveELISA, IndirectCompetitiveELISA, or FastELISA, automatically set to 0.01 (1:100).</div>
<div class="Bullet" id="326574593"><a name="326574593"></a>Pattern Description: Greater than or equal to 0 and less than or equal to 1 or Null.</div>
<div class="Bullet" id="1918466466"><a name="1918466466"></a>Programmatic Pattern: (RangeP[0, 1] | Automatic) | Null</div>
<div class="Bullet" id="1346418004"><a name="1346418004"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1212204789"></a><strong>PrimaryAntibodyVolume</strong></h4>
<div class="Text"><a name="348529634"></a>The volume of undiluted PrimaryAntibody added into the corresponding well of the assay plate. PrimaryAntibodyVolume is used as an alternative to PrimaryAntibodyDilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.</div>
<div class="Bullet" id="1549653269"><a name="1549653269"></a>Default Value: Null</div>
<div class="Bullet" id="340821267"><a name="340821267"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="844147920"><a name="844147920"></a>Programmatic Pattern: RangeP[0*Microliter, 200*Microliter] | Null</div>
<div class="Bullet" id="1317119358"><a name="1317119358"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="563616630"></a><strong>PrimaryAntibodyDiluent</strong></h4>
<div class="Text"><a name="154333493"></a>The buffer used to dilute the PrimaryAntibody.Set to Null when PrimaryAntibody should be diluted into samples (in DirectCompetitiveELISA, IndirectCompetitiveELISA, and FastELISA).</div>
<div class="Bullet" id="1870254922"><a name="1870254922"></a>Default Value: Automatic</div>
<div class="Bullet" id="656332915"><a name="656332915"></a>Default Calculation: If Method is set to DirectELISA, IndirectELISA, DirectSandwichELISA, IndirectSandwichELISA, the options will be automatically set to Model[Sample,"ELISA Blocker Blocking Buffer"].</div>
<div class="Bullet" id="1511291413"><a name="1511291413"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="1985685222"><a name="1985685222"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1515651774"></a><strong>PrimaryAntibodyStorageCondition</strong></h4>
<div class="Text"><a name="108344904"></a>The condition under which the unused portion of PrimaryAntibody stock sample should be stored.</div>
<div class="Bullet" id="2034152519"><a name="2034152519"></a>Default Value: Refrigerator</div>
<div class="Bullet" id="61726593"><a name="61726593"></a>Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, BacteriaIncubation, MammalianIncubation, TissueCultureCellsIncubation, MicrobialCellsIncubation, MicrobialCellsShakingIncubation, YeastCellsIncubation, YeastCellsShakingIncubation, ViralIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal.</div>
<div class="Bullet" id="204247050"><a name="204247050"></a>Programmatic Pattern: SampleStorageTypeP | Disposal</div>
<div class="Bullet" id="1101301444"><a name="1101301444"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="411815815"></a><strong>SecondaryAntibody</strong></h4>
<div class="Text"><a name="48608424"></a>The antibody that binds to the primary antibody.</div>
<div class="Bullet" id="917996308"><a name="917996308"></a>Default Value: Automatic</div>
<div class="Bullet" id="1241428508"><a name="1241428508"></a>Default Calculation: If Method is IndirectELISA, IndirectSandwichELISA, or IndirectCompetitiveELISA, the option is automatically set to a stocked secondary antibody for the primary antibody. </div>
<div class="Bullet" id="1885303351"><a name="1885303351"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="172208772"><a name="172208772"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<div class="Bullet" id="1652358495"><a name="1652358495"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="2078186108"></a><strong>SecondaryAntibodyDilutionFactor</strong></h4>
<div class="Text"><a name="1399882348"></a>The dilution ratio of SecondaryAntibody. SecondaryAntibody is always diluted in the SecondryAntibodyDiluent. Either SecondaryAntibodyDilutionFactor or SecondaryAntibodyVolume should be provided but not both.</div>
<div class="Bullet" id="2139269951"><a name="2139269951"></a>Default Value: Automatic</div>
<div class="Bullet" id="1538848383"><a name="1538848383"></a>Default Calculation: For IndirectELISA, IndirectSandwichELISA, IndirectCompetitiveELISA, automatically set to 0.001 (1:1,000).</div>
<div class="Bullet" id="1279212260"><a name="1279212260"></a>Pattern Description: Greater than or equal to 0 and less than or equal to 1 or Null.</div>
<div class="Bullet" id="1261663703"><a name="1261663703"></a>Programmatic Pattern: (RangeP[0, 1] | Automatic) | Null</div>
<div class="Bullet" id="528325843"><a name="528325843"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1875046603"></a><strong>SecondaryAntibodyVolume</strong></h4>
<div class="Text"><a name="1733220543"></a>The volume of SecondaryAntibody added into the corresponding well of the assay plate. SecondaryAntibodyVolume is used as an alternative to SecondaryAntibodyDilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.</div>
<div class="Bullet" id="1769478293"><a name="1769478293"></a>Default Value: Null</div>
<div class="Bullet" id="1268126795"><a name="1268126795"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="1779330737"><a name="1779330737"></a>Programmatic Pattern: RangeP[0*Microliter, 200*Microliter] | Null</div>
<div class="Bullet" id="1501912284"><a name="1501912284"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1116970350"></a><strong>SecondaryAntibodyDiluent</strong></h4>
<div class="Text"><a name="1766114023"></a>The buffer used to dilute SecondaryAntibody.</div>
<div class="Bullet" id="559415727"><a name="559415727"></a>Default Value: Automatic</div>
<div class="Bullet" id="416717123"><a name="416717123"></a>Default Calculation: If Method is set to IndirectELISA, IndirectSandwichELISA, IndirectCompetitiveELISA, the option will automatically set to Model[Sample,"ELISA Blocker Blocking Buffer"].</div>
<div class="Bullet" id="820513394"><a name="820513394"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="1376115571"><a name="1376115571"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="2123007254"></a><strong>SecondaryAntibodyStorageCondition</strong></h4>
<div class="Text"><a name="942123073"></a>The condition under which the unused portion of Secondary Antibody stock sample should be stored.</div>
<div class="Bullet" id="865558580"><a name="865558580"></a>Default Value: Automatic</div>
<div class="Bullet" id="388829282"><a name="388829282"></a>Default Calculation: If method is IndirectELISA, IndirectSandwichELISA, or IndirectCompetitiveELISA, the option is automatically set to Refrigerator.</div>
<div class="Bullet" id="261004753"><a name="261004753"></a>Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, BacteriaIncubation, MammalianIncubation, TissueCultureCellsIncubation, MicrobialCellsIncubation, MicrobialCellsShakingIncubation, YeastCellsIncubation, YeastCellsShakingIncubation, ViralIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.</div>
<div class="Bullet" id="1545276497"><a name="1545276497"></a>Programmatic Pattern: ((SampleStorageTypeP | Disposal) | Automatic) | Null</div>
<div class="Bullet" id="1942341908"><a name="1942341908"></a>Index Matches to: experiment samples</div>
<div class="ExLine"><a name="1041529709"></a><!-- --></div>
<h3 class="Subsubsection"><a name="850612466"></a>Sample Antibody Complex Incubation</h3>
<h4 class="Subsubsubsection"><a name="445077983"></a><strong>SampleAntibodyComplexIncubation</strong></h4>
<div class="Text"><a name="740117780"></a>Indicates if the pre-mixed samples and antibodies should be incubated before loaded into the assay plate. The plate used for sample-antibody mixing will be discarded after the experiment.</div>
<div class="Bullet" id="934245036"><a name="934245036"></a>Default Value: Automatic</div>
<div class="Bullet" id="1603376835"><a name="1603376835"></a>Default Calculation: Automatically set to True if Method is set to DirectCompetitiveELISA, IndirectCompetitiveELISA, and FastELISA.</div>
<div class="Bullet" id="1329663289"><a name="1329663289"></a>Pattern Description: {True, False}</div>
<div class="Bullet" id="936067541"><a name="936067541"></a>Programmatic Pattern: BooleanP | Automatic</div>
<h4 class="Subsubsubsection"><a name="21963665"></a><strong>SampleAntibodyComplexIncubationTime</strong></h4>
<div class="Text"><a name="1923614018"></a>The duration of sample-antibody complex incubation (If needed). In DirectCompetitiveELISA and IndirectCompetitiveELISA, PrimaryAntibody is incubated with the sample. In FastELISA, PrimaryAntibody and CaptureAntibody is incubated with the sample. If Null, the prepared sample-antibody complex will be kept at 4 degree Celsius till ready to use.</div>
<div class="Bullet" id="1961978588"><a name="1961978588"></a>Default Value: Automatic</div>
<div class="Bullet" id="362728831"><a name="362728831"></a>Default Calculation: If the Method is set to DirectCompetitiveELISA, IndirectCompetitiveELISA, or FastELISA, the option is automatically set to 2 Hours.</div>
<div class="Bullet" id="1824872431"><a name="1824872431"></a>Pattern Description: Greater than or equal to 0 minutes and less than or equal to 24 hours or Null.</div>
<div class="Bullet" id="269501363"><a name="269501363"></a>Programmatic Pattern: (RangeP[0*Minute, 24*Hour] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="466396418"></a><strong>SampleAntibodyComplexIncubationTemperature</strong></h4>
<div class="Text"><a name="409285776"></a>The temperature at which sample mixed with Antibodies are incubated. In DirectCompetitiveELISA and IndirectCompetitiveELISA, PrimaryAntibody is incubated with the sample. In FastELISA, PrimaryAntibody and CaptureAntibody are incubated with the sample. If Null, the prepared sample-antibody complex will be used directly.</div>
<div class="Bullet" id="475915891"><a name="475915891"></a>Default Value: Automatic</div>
<div class="Bullet" id="1489278609"><a name="1489278609"></a>Default Calculation: If the Method is set to DirectCompetitiveELISA, IndirectCompetitiveELISA, or FastELISA, the option is automatically set to Ambient.</div>
<div class="Bullet" id="1383675678"><a name="1383675678"></a>Pattern Description: Ambient or greater than or equal to 4 degrees Celsius and less than or equal to 50 degrees Celsius or Null.</div>
<div class="Bullet" id="336706783"><a name="336706783"></a>Programmatic Pattern: ((Ambient | RangeP[4*Celsius, 50*Celsius]) | Automatic) | Null</div>
<div class="ExLine"><a name="411492036"></a><!-- --></div>
<h3 class="Subsubsection"><a name="1049305712"></a>Coating</h3>
<h4 class="Subsubsubsection"><a name="545392420"></a><strong>Coating</strong></h4>
<div class="Text"><a name="950197544"></a>Indicates if Coating is required. Coating is a procedure to non-specifically adsorb protein molecules to the surface of wells of the assay plate.</div>
<div class="Bullet" id="1281722916"><a name="1281722916"></a>Default Value: True</div>
<div class="Bullet" id="508586155"><a name="508586155"></a>Pattern Description: {True, False}</div>
<div class="Bullet" id="822592025"><a name="822592025"></a>Programmatic Pattern: BooleanP</div>
<h4 class="Subsubsubsection"><a name="1951928436"></a><strong>SampleCoatingVolume</strong></h4>
<div class="Text"><a name="1101032280"></a>The amount of Sample that is aliquoted into the assay plate, in order for the Sample to be adsorbed to the surface of the well.</div>
<div class="Bullet" id="182943761"><a name="182943761"></a>Default Value: Automatic</div>
<div class="Bullet" id="1686692270"><a name="1686692270"></a>Default Calculation: If Method is DirectELISA or IndirectELISA, CoatingVolume is automatically set to 100 Microliter.</div>
<div class="Bullet" id="1452841490"><a name="1452841490"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="1017856040"><a name="1017856040"></a>Programmatic Pattern: (RangeP[0*Microliter, 200*Microliter] | Automatic) | Null</div>
<div class="Bullet" id="251732278"><a name="251732278"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="321611756"></a><strong>CoatingAntibodyCoatingVolume</strong></h4>
<div class="Text"><a name="112443593"></a>The amount of diluted CoatingAntibody that is aliquoted into the assay plate, in order for the CoatingAntibody to be adsorbed to the surface of the well.</div>
<div class="Bullet" id="53858191"><a name="53858191"></a>Default Value: Automatic</div>
<div class="Bullet" id="1104000750"><a name="1104000750"></a>Default Calculation: If Method is FastELISA, the option is automatically set to 100 Microliter.</div>
<div class="Bullet" id="681895170"><a name="681895170"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 300 microliters or Null.</div>
<div class="Bullet" id="1639381798"><a name="1639381798"></a>Programmatic Pattern: (RangeP[0*Microliter, 300*Microliter] | Automatic) | Null</div>
<div class="Bullet" id="874687976"><a name="874687976"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1355248917"></a><strong>ReferenceAntigenCoatingVolume</strong></h4>
<div class="Text"><a name="1456987937"></a>The amount of diluted ReferenceAntigen that is aliquoted into the assay plate, in order for the ReferenceAntigen to be adsorbed to the surface of the well.</div>
<div class="Bullet" id="1987714065"><a name="1987714065"></a>Default Value: Automatic</div>
<div class="Bullet" id="1254677723"><a name="1254677723"></a>Default Calculation: If Method is DirectCompetitiveELISA or IndirectCompetitiveELISA, CoatingVolume is automatically set to 100 Microliter.</div>
<div class="Bullet" id="1226560568"><a name="1226560568"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="1107938823"><a name="1107938823"></a>Programmatic Pattern: (RangeP[0*Microliter, 200*Microliter] | Automatic) | Null</div>
<div class="Bullet" id="297095024"><a name="297095024"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="376589093"></a><strong>CaptureAntibodyCoatingVolume</strong></h4>
<div class="Text"><a name="698578342"></a>The amount of diluted CaptureAntibody that is aliquoted into the assay plate, in order for the CaptureAntibody to be adsorbed to the surface of the well.</div>
<div class="Bullet" id="713095845"><a name="713095845"></a>Default Value: Automatic</div>
<div class="Bullet" id="2043116655"><a name="2043116655"></a>Default Calculation: If Method is DirectSandwichELISA or IndirectSandwichELISA, the option is automatically set to 100 Microliter.</div>
<div class="Bullet" id="398105055"><a name="398105055"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="1540098980"><a name="1540098980"></a>Programmatic Pattern: (RangeP[0*Microliter, 200*Microliter] | Automatic) | Null</div>
<div class="Bullet" id="823159569"><a name="823159569"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="753222209"></a><strong>CoatingTemperature</strong></h4>
<div class="Text"><a name="2137051245"></a>The temperature at which the Coating Solution is kept in the assay plate, in order for the coating molecules to be adsorbed to the surface of the well.</div>
<div class="Bullet" id="756278640"><a name="756278640"></a>Default Value: Automatic</div>
<div class="Bullet" id="1966879534"><a name="1966879534"></a>Default Calculation: If Coating is set to True, the option is automatically set to 4 degree Celsius.</div>
<div class="Bullet" id="1128549667"><a name="1128549667"></a>Pattern Description: Ambient or greater than or equal to 4 degrees Celsius and less than or equal to 50 degrees Celsius or Null.</div>
<div class="Bullet" id="958682965"><a name="958682965"></a>Programmatic Pattern: ((Ambient | RangeP[4*Celsius, 50*Celsius]) | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="14789314"></a><strong>CoatingTime</strong></h4>
<div class="Text"><a name="1603380993"></a>The duration when the Coating Solution is kept in the assay plate, in order for the coating molecules to be adsorbed to the surface of the well.</div>
<div class="Bullet" id="1399546795"><a name="1399546795"></a>Default Value: Automatic</div>
<div class="Bullet" id="794597974"><a name="794597974"></a>Default Calculation: If Coating is set to True, the option is automatically set to 16 Hours.</div>
<div class="Bullet" id="1754831972"><a name="1754831972"></a>Pattern Description: Greater than or equal to 0 hours and less than or equal to 20 hours or Null.</div>
<div class="Bullet" id="2068029153"><a name="2068029153"></a>Programmatic Pattern: (RangeP[0*Hour, 20*Hour] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="343147776"></a><strong>CoatingWashVolume</strong></h4>
<div class="Text"><a name="1291079037"></a>The volume of WashBuffer added to rinse off unbound molecule. When Coating is False but Blocking is True, the pre-coated plate must be washed at least once to ensure no liquid is left in the plate.</div>
<div class="Bullet" id="990605571"><a name="990605571"></a>Default Value: Automatic</div>
<div class="Bullet" id="1814600253"><a name="1814600253"></a>Default Calculation: If Coating is set to True, or Coating is False but Blocking is True, the option is automatically set to 250 Microliters.</div>
<div class="Bullet" id="1571181124"><a name="1571181124"></a>Pattern Description: Greater than or equal to 25 microliters and less than or equal to 300 microliters or Null.</div>
<div class="Bullet" id="1382227556"><a name="1382227556"></a>Programmatic Pattern: (RangeP[25*Microliter, 300*Microliter] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1767924093"></a><strong>CoatingNumberOfWashes</strong></h4>
<div class="Text"><a name="916491159"></a>The number of washes performed after coating. When Coating is False but Blocking is True, the pre-coated plate must be washed at least once to ensure no liquid is left in the plate.</div>
<div class="Bullet" id="1714193029"><a name="1714193029"></a>Default Value: Automatic</div>
<div class="Bullet" id="1949113898"><a name="1949113898"></a>Default Calculation: If Coating is set to True,or Coating is False but Blocking is True, the option is automatically set to 3.</div>
<div class="Bullet" id="1041732348"><a name="1041732348"></a>Pattern Description: Greater than 0 in increments of 1 or Null.</div>
<div class="Bullet" id="2108882492"><a name="2108882492"></a>Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null</div>
<div class="ExLine"><a name="1917932956"></a><!-- --></div>
<h3 class="Subsubsection"><a name="969650022"></a>Blocking</h3>
<h4 class="Subsubsubsection"><a name="1802006318"></a><strong>Blocking</strong></h4>
<div class="Text"><a name="358312985"></a>Indicates if a protein solution should be incubated with the assay plate to prevent non-specific binding of molecules to the assay plate.</div>
<div class="Bullet" id="622192707"><a name="622192707"></a>Default Value: True</div>
<div class="Bullet" id="1094949306"><a name="1094949306"></a>Pattern Description: {True, False}</div>
<div class="Bullet" id="1025614799"><a name="1025614799"></a>Programmatic Pattern: BooleanP</div>
<h4 class="Subsubsubsection"><a name="1804175971"></a><strong>BlockingBuffer</strong></h4>
<div class="Text"><a name="316448957"></a>The protein-containing solution used to prevent non-specific binding of antigen or antibody to the surface of the assay plate.</div>
<div class="Bullet" id="1388110327"><a name="1388110327"></a>Default Value: Automatic</div>
<div class="Bullet" id="1855408528"><a name="1855408528"></a>Default Calculation: If Blocking is True, automatically set to Model[Sample,"ELISA Blocker Blocking Buffer"].</div>
<div class="Bullet" id="241092009"><a name="241092009"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="1879237021"><a name="1879237021"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1294615518"></a><strong>BlockingVolume</strong></h4>
<div class="Text"><a name="298699622"></a>The amount of BlockingBuffer that is aliquoted into the appropriate wells of the assay plate, in order to prevent non-specific binding of molecules to the assay plate.</div>
<div class="Bullet" id="1575263915"><a name="1575263915"></a>Default Value: Automatic</div>
<div class="Bullet" id="1282219189"><a name="1282219189"></a>Default Calculation: If Blocking is True, the option is automatically set to 100 Microliters.</div>
<div class="Bullet" id="259511878"><a name="259511878"></a>Pattern Description: Greater than or equal to 25 microliters and less than or equal to 300 microliters or Null.</div>
<div class="Bullet" id="76846489"><a name="76846489"></a>Programmatic Pattern: (RangeP[25*Microliter, 300*Microliter] | Automatic) | Null</div>
<div class="Bullet" id="921268776"><a name="921268776"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="407223362"></a><strong>BlockingTime</strong></h4>
<div class="Text"><a name="172662145"></a>The duration when the BlockingBuffer is kept with the assay plate, in order to prevent non-specific binding of molecules to the assay plate.</div>
<div class="Bullet" id="682263918"><a name="682263918"></a>Default Value: Automatic</div>
<div class="Bullet" id="1394478493"><a name="1394478493"></a>Default Calculation: If Blocking is True, the option is automatically set to 1 Hour.</div>
<div class="Bullet" id="1510992140"><a name="1510992140"></a>Pattern Description: Greater than or equal to 0 hours and less than or equal to 20 hours or Null.</div>
<div class="Bullet" id="1250771205"><a name="1250771205"></a>Programmatic Pattern: (RangeP[0*Hour, 20*Hour] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="2141705599"></a><strong>BlockingTemperature</strong></h4>
<div class="Text"><a name="1672595026"></a>The duration of time when the BlockingBuffer is kept with the assay plate, in order to prevent non-specific binding of molecules to the assay plate.</div>
<div class="Bullet" id="743662752"><a name="743662752"></a>Default Value: Automatic</div>
<div class="Bullet" id="385047324"><a name="385047324"></a>Default Calculation: If Blocking is True, the option is automatically set to 25 degree Celsius.</div>
<div class="Bullet" id="1122146057"><a name="1122146057"></a>Pattern Description: Greater than or equal to 25 degrees Celsius and less than or equal to 50 degrees Celsius or Null.</div>
<div class="Bullet" id="707392045"><a name="707392045"></a>Programmatic Pattern: (RangeP[25*Celsius, 50*Celsius] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="668630523"></a><strong>BlockingMixRate</strong></h4>
<div class="Text"><a name="2038758957"></a>The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during Blocking incubation. Mixing is not recommended when incubation volume is high, in which case the option should be set to Null.</div>
<div class="Bullet" id="172718767"><a name="172718767"></a>Default Value: Null</div>
<div class="Bullet" id="1633909872"><a name="1633909872"></a>Pattern Description: Greater than or equal to 40 revolutions per minute and less than or equal to 1000 revolutions per minute or Null.</div>
<div class="Bullet" id="1249824515"><a name="1249824515"></a>Programmatic Pattern: RangeP[40*RPM, 1000*RPM] | Null</div>
<h4 class="Subsubsubsection"><a name="1263072298"></a><strong>BlockingWashVolume</strong></h4>
<div class="Text"><a name="580261891"></a>The volume of WashBuffer added after Blocking, in order to rinse off the unbound molecules from the surface of the wells. If Coating and Blocking are both False, at least one blocking wash is required to ensure no liquid is remaining in the assay plate.</div>
<div class="Bullet" id="738361010"><a name="738361010"></a>Default Value: Automatic</div>
<div class="Bullet" id="1472982704"><a name="1472982704"></a>Default Calculation: If Blocking is True, or Coating and Blocking are both False, the option is automatically set to 250 Microliters.</div>
<div class="Bullet" id="228823512"><a name="228823512"></a>Pattern Description: Greater than or equal to 25 microliters and less than or equal to 300 microliters or Null.</div>
<div class="Bullet" id="1841038054"><a name="1841038054"></a>Programmatic Pattern: (RangeP[25*Microliter, 300*Microliter] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1382187602"></a><strong>BlockingNumberOfWashes</strong></h4>
<div class="Text"><a name="1096417215"></a>The number of washes performed after Blocking, in order to rinse off the unbound molecules from the surface of the wells.</div>
<div class="Bullet" id="2074441245"><a name="2074441245"></a>Default Value: Automatic</div>
<div class="Bullet" id="736995670"><a name="736995670"></a>Default Calculation: If Blocking is True, or Coating and Blocking are both False, the option is automatically set to 3.</div>
<div class="Bullet" id="549794"><a name="549794"></a>Pattern Description: Greater than 0 in increments of 1 or Null.</div>
<div class="Bullet" id="650453170"><a name="650453170"></a>Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null</div>
<div class="ExLine"><a name="1474664960"></a><!-- --></div>
<h3 class="Subsubsection"><a name="585212693"></a>Immunosorbent Step</h3>
<h4 class="Subsubsubsection"><a name="194627991"></a><strong>SampleAntibodyComplexImmunosorbentVolume</strong></h4>
<div class="Text"><a name="495050356"></a>The volume of the sample-antibody complex to be loaded on each well of the ELISAPlate. In DirectCompetitiveELISA and IndirectCompetitiveELISA, this step enables the free primary antibody to bind to the ReferenceAntigen coated on the plate. In FastELISA, this step enables the PrimaryAntibody-TargetAntigen-CaptureAntibody complex to bind to the CoatingAntibody on the plate.</div>
<div class="Bullet" id="959684814"><a name="959684814"></a>Default Value: Automatic</div>
<div class="Bullet" id="1820479928"><a name="1820479928"></a>Default Calculation: If the Method is set to DirectCompetitiveELISA, IndirectCompetitiveELISA, or FastELISA, the option is automatically set to 100 Microliter.</div>
<div class="Bullet" id="1606631087"><a name="1606631087"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="189301831"><a name="189301831"></a>Programmatic Pattern: (RangeP[0*Microliter, 200*Microliter] | Automatic) | Null</div>
<div class="Bullet" id="1172592410"><a name="1172592410"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="303206351"></a><strong>SampleAntibodyComplexImmunosorbentTime</strong></h4>
<div class="Text"><a name="10446926"></a>The duration of sample-antibody complex incubation.</div>
<div class="Bullet" id="1635309875"><a name="1635309875"></a>Default Value: Automatic</div>
<div class="Bullet" id="1157354819"><a name="1157354819"></a>Default Calculation: If the Method is set to DirectCompetitiveELISA, IndirectCompetitiveELISA, or FastELISA, the option is automatically set to 2 Hour.</div>
<div class="Bullet" id="1903052054"><a name="1903052054"></a>Pattern Description: Greater than or equal to 0 minutes and less than or equal to 24 hours or Null.</div>
<div class="Bullet" id="2121916807"><a name="2121916807"></a>Programmatic Pattern: (RangeP[0*Minute, 24*Hour] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1942333167"></a><strong>SampleAntibodyComplexImmunosorbentTemperature</strong></h4>
<div class="Text"><a name="894619722"></a>The temperature of the sample-antibody complex incubation.</div>
<div class="Bullet" id="1340655007"><a name="1340655007"></a>Default Value: Automatic</div>
<div class="Bullet" id="990278325"><a name="990278325"></a>Default Calculation: If the Method is set to DirectCompetitiveELISA, IndirectCompetitiveELISA, or FastELISA, the option is automatically set to 25 Celsius.</div>
<div class="Bullet" id="609544025"><a name="609544025"></a>Pattern Description: Ambient or greater than or equal to 4 degrees Celsius and less than or equal to 50 degrees Celsius or Null.</div>
<div class="Bullet" id="1109431985"><a name="1109431985"></a>Programmatic Pattern: ((Ambient | RangeP[4*Celsius, 50*Celsius]) | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1770348641"></a><strong>SampleAntibodyComplexImmunosorbentMixRate</strong></h4>
<div class="Text"><a name="863680102"></a>The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during SampleAntibody mixture incubation in the assay plate. Mixing is not recommended when incubation volume is higher than 200 Microliters, in which case the options should be set to Null.</div>
<div class="Bullet" id="1029504241"><a name="1029504241"></a>Default Value: Null</div>
<div class="Bullet" id="602034608"><a name="602034608"></a>Pattern Description: Greater than or equal to 40 revolutions per minute and less than or equal to 1000 revolutions per minute or Null.</div>
<div class="Bullet" id="1600195639"><a name="1600195639"></a>Programmatic Pattern: RangeP[40*RPM, 1000*RPM] | Null</div>
<h4 class="Subsubsubsection"><a name="1550393292"></a><strong>SampleAntibodyComplexImmunosorbentWashVolume</strong></h4>
<div class="Text"><a name="2040969593"></a>The volume of WashBuffer added to rinse off the unbound primary antibody after sample-antibody complex incubation.</div>
<div class="Bullet" id="819656020"><a name="819656020"></a>Default Value: Automatic</div>
<div class="Bullet" id="1998616282"><a name="1998616282"></a>Default Calculation: If the Method is set to DirectCompetitiveELISA, IndirectCompetitiveELISA, or FastELISA, the option is automatically set to 250 Microliter.</div>
<div class="Bullet" id="1952128847"><a name="1952128847"></a>Pattern Description: Greater than or equal to 25 microliters and less than or equal to 300 microliters or Null.</div>
<div class="Bullet" id="174372663"><a name="174372663"></a>Programmatic Pattern: (RangeP[25*Microliter, 300*Microliter] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1513652533"></a><strong>SampleAntibodyComplexImmunosorbentNumberOfWashes</strong></h4>
<div class="Text"><a name="866839769"></a>The number of rinses performed after sample-antibody complex incubation.</div>
<div class="Bullet" id="446936335"><a name="446936335"></a>Default Value: Automatic</div>
<div class="Bullet" id="1908668786"><a name="1908668786"></a>Default Calculation: If the Method is set to DirectCompetitiveELISA, IndirectCompetitiveELISA, or FastELISA, the option is automatically set to 4.</div>
<div class="Bullet" id="2033051063"><a name="2033051063"></a>Pattern Description: Greater than 0 in increments of 1 or Null.</div>
<div class="Bullet" id="876908424"><a name="876908424"></a>Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="19612807"></a><strong>SampleImmunosorbentVolume</strong></h4>
<div class="Text"><a name="1067449258"></a>The volume of the Sample to be loaded on the ELISAPlate for the target antigen to bind to the capture antibody in DirectSandwichELISA and IndirectSandwichELISA.</div>
<div class="Bullet" id="541292168"><a name="541292168"></a>Default Value: Automatic</div>
<div class="Bullet" id="756738884"><a name="756738884"></a>Default Calculation: If the Method is set to DirectSandwichELISA and IndirectSandwichELISA, the option is automatically set to 100 Microliters.</div>
<div class="Bullet" id="1112265854"><a name="1112265854"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="7061043"><a name="7061043"></a>Programmatic Pattern: (RangeP[0*Microliter, 200*Microliter] | Automatic) | Null</div>
<div class="Bullet" id="563349116"><a name="563349116"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="2100676636"></a><strong>SampleImmunosorbentTime</strong></h4>
<div class="Text"><a name="1441064572"></a>The duration of Sample incubation.</div>
<div class="Bullet" id="651690738"><a name="651690738"></a>Default Value: Automatic</div>
<div class="Bullet" id="799633866"><a name="799633866"></a>Default Calculation: If the Method is set to DirectSandwichELISA and IndirectSandwichELISA, the option is automatically set to 2 Hour.</div>
<div class="Bullet" id="493722936"><a name="493722936"></a>Pattern Description: Greater than or equal to 0 minutes and less than or equal to 24 hours or Null.</div>
<div class="Bullet" id="124573344"><a name="124573344"></a>Programmatic Pattern: (RangeP[0*Minute, 24*Hour] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="2055120430"></a><strong>SampleImmunosorbentTemperature</strong></h4>
<div class="Text"><a name="282088662"></a>The temperature of the Sample incubation.</div>
<div class="Bullet" id="1567733305"><a name="1567733305"></a>Default Value: Automatic</div>
<div class="Bullet" id="1416792092"><a name="1416792092"></a>Default Calculation: If the Method is set to DirectSandwichELISA and IndirectSandwichELISA, the option is automatically set to 25 Celsius.</div>
<div class="Bullet" id="726012308"><a name="726012308"></a>Pattern Description: Greater than or equal to 25 degrees Celsius and less than or equal to 50 degrees Celsius or Null.</div>
<div class="Bullet" id="86778302"><a name="86778302"></a>Programmatic Pattern: (RangeP[25*Celsius, 50*Celsius] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="341525401"></a><strong>SampleImmunosorbentMixRate</strong></h4>
<div class="Text"><a name="1941109823"></a>The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during Sample incubation. Mixing is not recommended when incubation volume is higher than 200 Microliters, in which case the options should be set to Null.</div>
<div class="Bullet" id="1808713584"><a name="1808713584"></a>Default Value: Null</div>
<div class="Bullet" id="1418183003"><a name="1418183003"></a>Pattern Description: Greater than or equal to 40 revolutions per minute and less than or equal to 1000 revolutions per minute or Null.</div>
<div class="Bullet" id="480733368"><a name="480733368"></a>Programmatic Pattern: RangeP[40*RPM, 1000*RPM] | Null</div>
<h4 class="Subsubsubsection"><a name="852235962"></a><strong>SampleImmunosorbentWashVolume</strong></h4>
<div class="Text"><a name="1961371491"></a>The volume of WashBuffer added to rinse off the unbound Sample after Sample incubation.</div>
<div class="Bullet" id="896667787"><a name="896667787"></a>Default Value: Automatic</div>
<div class="Bullet" id="1402745110"><a name="1402745110"></a>Default Calculation: If the Method is set to DirectSandwichELISA and IndirectSandwichELISA, the option is automatically set to 250 Microliters.</div>
<div class="Bullet" id="861587004"><a name="861587004"></a>Pattern Description: Greater than or equal to 25 microliters and less than or equal to 300 microliters or Null.</div>
<div class="Bullet" id="210544507"><a name="210544507"></a>Programmatic Pattern: (RangeP[25*Microliter, 300*Microliter] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1715962540"></a><strong>SampleImmunosorbentNumberOfWashes</strong></h4>
<div class="Text"><a name="1624514217"></a>The number of rinses performed after Sample incubation.</div>
<div class="Bullet" id="103357161"><a name="103357161"></a>Default Value: Automatic</div>
<div class="Bullet" id="1957018151"><a name="1957018151"></a>Default Calculation: If the Method is set to DirectSandwichELISA and IndirectSandwichELISA, the option is automatically set to 4.</div>
<div class="Bullet" id="744526405"><a name="744526405"></a>Pattern Description: Greater than 0 in increments of 1 or Null.</div>
<div class="Bullet" id="2015561413"><a name="2015561413"></a>Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1133620513"></a><strong>PrimaryAntibodyImmunosorbentVolume</strong></h4>
<div class="Text"><a name="285045807"></a>The volume of the PrimaryAntibody to be loaded on the ELISA Plate for immunosorbent step.</div>
<div class="Bullet" id="1876345439"><a name="1876345439"></a>Default Value: Automatic</div>
<div class="Bullet" id="2087920725"><a name="2087920725"></a>Default Calculation: If</div>
<div class="Bullet" id="1800833095"><a name="1800833095"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="2114790494"><a name="2114790494"></a>Programmatic Pattern: (RangeP[0*Microliter, 200*Microliter] | Automatic) | Null</div>
<div class="Bullet" id="281991161"><a name="281991161"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="2076517645"></a><strong>PrimaryAntibodyImmunosorbentTime</strong></h4>
<div class="Text"><a name="1275312118"></a>The duration of PrimaryAntibody incubation.</div>
<div class="Bullet" id="136486519"><a name="136486519"></a>Default Value: Automatic</div>
<div class="Bullet" id="416389837"><a name="416389837"></a>Default Calculation: If the Method is set to DirectELISA, IndirectELISA, DirectSandwichELISA, or IndirectSandwichELISA, the option is automatically set to 2 Hour.</div>
<div class="Bullet" id="1762268533"><a name="1762268533"></a>Pattern Description: Greater than or equal to 0 minutes and less than or equal to 24 hours or Null.</div>
<div class="Bullet" id="352774707"><a name="352774707"></a>Programmatic Pattern: (RangeP[0*Minute, 24*Hour] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="2029634829"></a><strong>PrimaryAntibodyImmunosorbentTemperature</strong></h4>
<div class="Text"><a name="1442322055"></a>The temperature of the PrimaryAntibody incubation.</div>
<div class="Bullet" id="311371049"><a name="311371049"></a>Default Value: Automatic</div>
<div class="Bullet" id="1943056451"><a name="1943056451"></a>Default Calculation: If the Method is set to DirectELISA, IndirectELISA, DirectSandwichELISA, or IndirectSandwichELISA, the option is automatically set to 25 Celsius.</div>
<div class="Bullet" id="165952028"><a name="165952028"></a>Pattern Description: Greater than or equal to 25 degrees Celsius and less than or equal to 50 degrees Celsius or Null.</div>
<div class="Bullet" id="1721960790"><a name="1721960790"></a>Programmatic Pattern: (RangeP[25*Celsius, 50*Celsius] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1505370558"></a><strong>PrimaryAntibodyImmunosorbentMixRate</strong></h4>
<div class="Text"><a name="1258122999"></a>The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during PrimaryAntibody incubation. Mixing is not recommended when incubation volume is higher than 200 Microliters, in which case the options should be set to Null.</div>
<div class="Bullet" id="1149255831"><a name="1149255831"></a>Default Value: Null</div>
<div class="Bullet" id="1074830499"><a name="1074830499"></a>Pattern Description: Greater than or equal to 40 revolutions per minute and less than or equal to 1000 revolutions per minute or Null.</div>
<div class="Bullet" id="43758129"><a name="43758129"></a>Programmatic Pattern: RangeP[40*RPM, 1000*RPM] | Null</div>
<h4 class="Subsubsubsection"><a name="1003466829"></a><strong>PrimaryAntibodyImmunosorbentWashVolume</strong></h4>
<div class="Text"><a name="1077915112"></a>The volume of WashBuffer added to rinse off the unbound primary antibody after PrimaryAntibody incubation.</div>
<div class="Bullet" id="347241292"><a name="347241292"></a>Default Value: Automatic</div>
<div class="Bullet" id="1371325745"><a name="1371325745"></a>Default Calculation: If the Method is set to DirectELISA, IndirectELISA, DirectSandwichELISA, or IndirectSandwichELISA, the option is automatically set to 250 Microliters.</div>
<div class="Bullet" id="1077296611"><a name="1077296611"></a>Pattern Description: Greater than or equal to 25 microliters and less than or equal to 300 microliters or Null.</div>
<div class="Bullet" id="689513220"><a name="689513220"></a>Programmatic Pattern: (RangeP[25*Microliter, 300*Microliter] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="826929328"></a><strong>PrimaryAntibodyImmunosorbentNumberOfWashes</strong></h4>
<div class="Text"><a name="1834535959"></a>The number of rinses performed after PrimaryAntibody incubation.</div>
<div class="Bullet" id="1623142934"><a name="1623142934"></a>Default Value: Automatic</div>
<div class="Bullet" id="678523897"><a name="678523897"></a>Default Calculation: If the Method is set to DirectELISA, IndirectELISA, DirectSandwichELISA, or IndirectSandwichELISA, the option is automatically set to 4.</div>
<div class="Bullet" id="812971309"><a name="812971309"></a>Pattern Description: Greater than 0 in increments of 1 or Null.</div>
<div class="Bullet" id="1317828149"><a name="1317828149"></a>Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1738848732"></a><strong>SecondaryAntibodyImmunosorbentVolume</strong></h4>
<div class="Text"><a name="1873170348"></a>The volume of the Secondary Antibody to be loaded on the ELISAPlate for the immunosorbent step.</div>
<div class="Bullet" id="263773816"><a name="263773816"></a>Default Value: Automatic</div>
<div class="Bullet" id="840278104"><a name="840278104"></a>Default Calculation: If the Method is set to IndirectELISA, IndirectSandwichELISA, and IndirectCompetitiveELISA, the option is automatically set to 100 Microliter.</div>
<div class="Bullet" id="701631256"><a name="701631256"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="483813915"><a name="483813915"></a>Programmatic Pattern: (RangeP[0*Microliter, 200*Microliter] | Automatic) | Null</div>
<div class="Bullet" id="1087381863"><a name="1087381863"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="541135471"></a><strong>SecondaryAntibodyImmunosorbentTime</strong></h4>
<div class="Text"><a name="270616052"></a>The duration of Secondary Antibody incubation.</div>
<div class="Bullet" id="2021105265"><a name="2021105265"></a>Default Value: Automatic</div>
<div class="Bullet" id="1967344256"><a name="1967344256"></a>Default Calculation: If the Method is set to IndirectELISA, IndirectSandwichELISA, and IndirectCompetitiveELISA, the option is automatically set to 1 Hour.</div>
<div class="Bullet" id="349197733"><a name="349197733"></a>Pattern Description: Greater than or equal to 0 minutes and less than or equal to 24 hours or Null.</div>
<div class="Bullet" id="2040974927"><a name="2040974927"></a>Programmatic Pattern: (RangeP[0*Minute, 24*Hour] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="909304558"></a><strong>SecondaryAntibodyImmunosorbentTemperature</strong></h4>
<div class="Text"><a name="1188074254"></a>The temperature of the Secondary Antibody incubation.</div>
<div class="Bullet" id="661037172"><a name="661037172"></a>Default Value: Automatic</div>
<div class="Bullet" id="1118843873"><a name="1118843873"></a>Default Calculation: If the Method is set to IndirectELISA, IndirectSandwichELISA, and IndirectCompetitiveELISA, the option is automatically set to 25 Celsius.</div>
<div class="Bullet" id="1042160379"><a name="1042160379"></a>Pattern Description: Greater than or equal to 25 degrees Celsius and less than or equal to 50 degrees Celsius or Null.</div>
<div class="Bullet" id="712864921"><a name="712864921"></a>Programmatic Pattern: (RangeP[25*Celsius, 50*Celsius] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="309460634"></a><strong>SecondaryAntibodyImmunosorbentMixRate</strong></h4>
<div class="Text"><a name="2046966251"></a>The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during Secondary Antibody incubation. Mixing is not recommended when incubation volume is higher than 200 Microliters, in which case the options should be set to Null.</div>
<div class="Bullet" id="673755617"><a name="673755617"></a>Default Value: Null</div>
<div class="Bullet" id="129384288"><a name="129384288"></a>Pattern Description: Greater than or equal to 40 revolutions per minute and less than or equal to 1000 revolutions per minute or Null.</div>
<div class="Bullet" id="1308277652"><a name="1308277652"></a>Programmatic Pattern: RangeP[40*RPM, 1000*RPM] | Null</div>
<h4 class="Subsubsubsection"><a name="137435531"></a><strong>SecondaryAntibodyImmunosorbentWashVolume</strong></h4>
<div class="Text"><a name="1334048992"></a>The volume of WashBuffer added to rinse off the unbound Secondary antibody after SecondaryAntibody incubation.</div>
<div class="Bullet" id="1632133864"><a name="1632133864"></a>Default Value: Automatic</div>
<div class="Bullet" id="1465229117"><a name="1465229117"></a>Default Calculation: If the Method is set to IndirectELISA, IndirectSandwichELISA, and IndirectCompetitiveELISA, the option is automatically set to 250 Microliters.</div>
<div class="Bullet" id="910789270"><a name="910789270"></a>Pattern Description: Greater than or equal to 25 microliters and less than or equal to 300 microliters or Null.</div>
<div class="Bullet" id="371825074"><a name="371825074"></a>Programmatic Pattern: (RangeP[25*Microliter, 300*Microliter] | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="86605948"></a><strong>SecondaryAntibodyImmunosorbentNumberOfWashes</strong></h4>
<div class="Text"><a name="1739739017"></a>The number of rinses performed after SecondaryAntibody incubation.</div>
<div class="Bullet" id="1803804814"><a name="1803804814"></a>Default Value: Automatic</div>
<div class="Bullet" id="520864199"><a name="520864199"></a>Default Calculation: If the Method is set to IndirectELISA, IndirectSandwichELISA, and IndirectCompetitiveELISA, the option is automatically set to 4.</div>
<div class="Bullet" id="1021247421"><a name="1021247421"></a>Pattern Description: Greater than 0 in increments of 1 or Null.</div>
<div class="Bullet" id="1416097923"><a name="1416097923"></a>Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null</div>
<div class="ExLine"><a name="1944015807"></a><!-- --></div>
<h3 class="Subsubsection"><a name="1257462791"></a>Detection</h3>
<h4 class="Subsubsubsection"><a name="790558210"></a><strong>SubstrateSolution</strong></h4>
<div class="Text"><a name="430511481"></a>Defines the substrate solution to the enzyme conjugated to the antibody.</div>
<div class="Bullet" id="734054424"><a name="734054424"></a>Default Value: Automatic</div>
<div class="Bullet" id="2106635800"><a name="2106635800"></a>Default Calculation: If enzyme is Horseredish Peroxidase, the option will be automatically set to Model[Sample,"ELISA TMB Stabilized Chromogen"]. If enzyme is Alkaline Phosphatase, then the option is automatically set to Model[Sample,"AP Substrate PNPP Solution"].</div>
<div class="Bullet" id="665002511"><a name="665002511"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample.</div>
<div class="Bullet" id="1192303389"><a name="1192303389"></a>Programmatic Pattern: (ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic</div>
<div class="Bullet" id="873148766"><a name="873148766"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1255550211"></a><strong>StopSolution</strong></h4>
<div class="Text"><a name="858080855"></a>The reagent that is used to stop the reaction between the enzyme and its substrate.</div>
<div class="Bullet" id="1412240380"><a name="1412240380"></a>Default Value: Automatic</div>
<div class="Bullet" id="1534800016"><a name="1534800016"></a>Default Calculation: If enzyme is Horseradish Peroxidase, then the option is automatically set to Model[Sample,"ELISA HRP-TMB Stop Solution"]. If enzyme is Alkaline Phosphatase, then the option is automatically set to Model[Sample,"AP Substrate Stop Solution"].</div>
<div class="Bullet" id="1957784795"><a name="1957784795"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="744610231"><a name="744610231"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<div class="Bullet" id="1276941348"><a name="1276941348"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1749151365"></a><strong>SubstrateSolutionVolume</strong></h4>
<div class="Text"><a name="1083347772"></a>The volume of substrate to be added to the corresponding well.</div>
<div class="Bullet" id="1459644738"><a name="1459644738"></a>Default Value: Automatic</div>
<div class="Bullet" id="1543411885"><a name="1543411885"></a>Default Calculation: If SubstrateSolution is populated, then the option is automatically set to 100ul.</div>
<div class="Bullet" id="668579082"><a name="668579082"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters.</div>
<div class="Bullet" id="1174190070"><a name="1174190070"></a>Programmatic Pattern: RangeP[0*Microliter, 200*Microliter] | Automatic</div>
<div class="Bullet" id="1385274207"><a name="1385274207"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="1433379922"></a><strong>StopSolutionVolume</strong></h4>
<div class="Text"><a name="344797008"></a>The volume of StopSolution to be added to the corresponding well.</div>
<div class="Bullet" id="1092433850"><a name="1092433850"></a>Default Value: Automatic</div>
<div class="Bullet" id="1698018747"><a name="1698018747"></a>Default Calculation: If StopSolution is selected, the StopSolutionVolume is automatically set to 100ul.</div>
<div class="Bullet" id="690895846"><a name="690895846"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="442404393"><a name="442404393"></a>Programmatic Pattern: (RangeP[0*Microliter, 200*Microliter] | Automatic) | Null</div>
<div class="Bullet" id="902247237"><a name="902247237"></a>Index Matches to: experiment samples</div>
<h4 class="Subsubsubsection"><a name="687280792"></a><strong>SubstrateIncubationTime</strong></h4>
<div class="Text"><a name="1961217578"></a>The time during which the the colorimetric reaction occurs.</div>
<div class="Bullet" id="457335643"><a name="457335643"></a>Default Value: 30 minutes</div>
<div class="Bullet" id="596179288"><a name="596179288"></a>Pattern Description: Greater than or equal to 0 minutes and less than or equal to 24 hours.</div>
<div class="Bullet" id="1974080161"><a name="1974080161"></a>Programmatic Pattern: RangeP[0*Minute, 24*Hour]</div>
<h4 class="Subsubsubsection"><a name="1890403424"></a><strong>SubstrateIncubationTemperature</strong></h4>
<div class="Text"><a name="2137273450"></a>The temperature of the Substrate incubation, in order for the detection reaction to take place where the antibody-conjugated enzyme (such as Horseradish Peroxidase or Alkaline Phosphatase) catalyzes the colorimetric reaction.</div>
<div class="Bullet" id="195910781"><a name="195910781"></a>Default Value: 25 degrees Celsius</div>
<div class="Bullet" id="580065416"><a name="580065416"></a>Pattern Description: Greater than or equal to 25 degrees Celsius and less than or equal to 50 degrees Celsius.</div>
<div class="Bullet" id="1731172979"><a name="1731172979"></a>Programmatic Pattern: RangeP[25*Celsius, 50*Celsius]</div>
<h4 class="Subsubsubsection"><a name="1715808497"></a><strong>SubstrateIncubationMixRate</strong></h4>
<div class="Text"><a name="1182997163"></a>The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during Substrate incubation. Mixing is not recommended when incubation volume is higher than 200 Microliters, in which case the options should be set to Null.</div>
<div class="Bullet" id="1229714615"><a name="1229714615"></a>Default Value: Null</div>
<div class="Bullet" id="430915577"><a name="430915577"></a>Pattern Description: Greater than or equal to 40 revolutions per minute and less than or equal to 1000 revolutions per minute or Null.</div>
<div class="Bullet" id="1083244955"><a name="1083244955"></a>Programmatic Pattern: RangeP[40*RPM, 1000*RPM] | Null</div>
<h4 class="Subsubsubsection"><a name="1879083066"></a><strong>AbsorbanceWavelength</strong></h4>
<div class="Text"><a name="854577480"></a>The wavelength used to detect the absorbance of light by the product of the detection reaction.</div>
<div class="Bullet" id="513075224"><a name="513075224"></a>Default Value: 450 nanometers</div>
<div class="Bullet" id="1108447063"><a name="1108447063"></a>Pattern Description: {405 nanometers, 450 nanometers, 492 nanometers, 620 nanometers} or list of one or more {405 nanometers, 450 nanometers, 492 nanometers, 620 nanometers} entries.</div>
<div class="Bullet" id="249150116"><a name="249150116"></a>Programmatic Pattern: (405*Nanometer | 450*Nanometer | 492*Nanometer | 620*Nanometer) | {(405*Nanometer | 450*Nanometer | 492*Nanometer | 620*Nanometer)..}</div>
<h4 class="Subsubsubsection"><a name="2020371609"></a><strong>PrereadBeforeStop</strong></h4>
<div class="Text"><a name="374206099"></a>Indicate if colorimetric reactions between the enzyme and its substrate will be checked by the plate reader before the stopping reagent was added to terminate the reaction.</div>
<div class="Bullet" id="1449787477"><a name="1449787477"></a>Default Value: Automatic</div>
<div class="Bullet" id="1228667077"><a name="1228667077"></a>Default Calculation: Is set automatically to True if either or both of PrereadTimepoints and PrereadAbsorbanceWavelength are specified. Otherwise is set to False.</div>
<div class="Bullet" id="4813587"><a name="4813587"></a>Pattern Description: True or False.</div>
<div class="Bullet" id="1445061770"><a name="1445061770"></a>Programmatic Pattern: BooleanP | Automatic</div>
<h4 class="Subsubsubsection"><a name="1260604467"></a><strong>PrereadTimepoints</strong></h4>
<div class="Text"><a name="2053099214"></a>The list of time points when absorbance intensitied at each PreparedAbsorbanceWavelength during the Preread process (before the colorimetric reaction was terminated).</div>
<div class="Bullet" id="671249702"><a name="671249702"></a>Default Value: Automatic</div>
<div class="Bullet" id="962143823"><a name="962143823"></a>Default Calculation: If PrereadBeforeStop is True, this option is set to one single value: the half of the SubstrateIncubationTime. Otherwise is set to Null.</div>
<div class="Bullet" id="199371251"><a name="199371251"></a>Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or list of one or more greater than or equal to 0 minutes and less than or equal to 72 hours entries or Null.</div>
<div class="Bullet" id="758126237"><a name="758126237"></a>Programmatic Pattern: ((RangeP[0*Minute, $MaxExperimentTime] | {RangeP[0*Minute, $MaxExperimentTime]..}) | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="807187608"></a><strong>PrereadAbsorbanceWavelength</strong></h4>
<div class="Text"><a name="747929557"></a>The wavelength used to detect the absorbance of light during the Preread process (before the colorimetric reaction was terminated).</div>
<div class="Bullet" id="1230278608"><a name="1230278608"></a>Default Value: Automatic</div>
<div class="Bullet" id="1320011340"><a name="1320011340"></a>Default Calculation: If PrereadBeforeStop is True, this option is set to {450 Nanometer}. Otherwise is set to Null.</div>
<div class="Bullet" id="1924517870"><a name="1924517870"></a>Pattern Description: {405 nanometers, 450 nanometers, 492 nanometers, 620 nanometers} or list of one or more {405 nanometers, 450 nanometers, 492 nanometers, 620 nanometers} entries or Null.</div>
<div class="Bullet" id="2120633623"><a name="2120633623"></a>Programmatic Pattern: (((405*Nanometer | 450*Nanometer | 492*Nanometer | 620*Nanometer) | {(405*Nanometer | 450*Nanometer | 492*Nanometer | 620*Nanometer)..}) | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="1850696149"></a><strong>SignalCorrection</strong></h4>
<div class="Text"><a name="497033095"></a>The absorbance reading that is used to eliminate the interference of background absorbance (such as from ELISAPlate material and dust). If True, a reading at 620 nm is read at the same time of the AbsorbanceWavelength. The correction is done by subtracting the reading at 620nm from that at the AbsorbanceWavelength.</div>
<div class="Bullet" id="2071324482"><a name="2071324482"></a>Default Value: False</div>
<div class="Bullet" id="2040651104"><a name="2040651104"></a>Pattern Description: {True, False} or Null.</div>
<div class="Bullet" id="1909262338"><a name="1909262338"></a>Programmatic Pattern: BooleanP | Null</div>
<div class="ExLine"><a name="1271461292"></a><!-- --></div>
<h3 class="Subsubsection"><a name="1987646994"></a>Standard</h3>
<h4 class="Subsubsubsection"><a name="127415426"></a><strong>Standard</strong></h4>
<div class="Text"><a name="429868723"></a>A sample containing known amount of TargetAntigen molecule. Standard is used for the quantification of Standard analyte.</div>
<div class="Bullet" id="668638953"><a name="668638953"></a>Default Value: Automatic</div>
<div class="Bullet" id="32958320"><a name="32958320"></a>Default Calculation: If TargetAntigen is specified, standard will be automatically set to the DefaultSampleModel of the target antigen.</div>
<div class="Bullet" id="2027186961"><a name="2027186961"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="1103193872"><a name="1103193872"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<div class="Bullet" id="5598506"><a name="5598506"></a>Index Matches to: Standard</div>
<h4 class="Subsubsubsection"><a name="1752293521"></a><strong>StandardTargetAntigen</strong></h4>
<div class="Text"><a name="206472489"></a>The Analyte molecule(e.g., peptide, protein, and hormone) detected and quantified in the Standard samples by Antibodies in the ELISA experiment. This option is used to automatically set sample Antibodies and the corresponding experiment conditions of Standards and Blanks.</div>
<div class="Bullet" id="1997032718"><a name="1997032718"></a>Default Value: Automatic</div>
<div class="Bullet" id="1106972463"><a name="1106972463"></a>Default Calculation: Automatically set to the Model[Molecule] in the Analyte field of the sample.</div>
<div class="Bullet" id="1235351680"><a name="1235351680"></a>Pattern Description: An object of type or subtype Model[Molecule] or Null.</div>
<div class="Bullet" id="683786564"><a name="683786564"></a>Programmatic Pattern: (ObjectP[{Model[Molecule]}] | Automatic) | Null</div>
<div class="Bullet" id="1215786051"><a name="1215786051"></a>Index Matches to: Standard</div>
<h4 class="Subsubsubsection"><a name="409257952"></a><strong>StandardSerialDilutionCurve</strong></h4>
<div class="Text"><a name="8277923"></a>The collection of serial dilutions that will be performed on sample. StandardLoadingVolume of each dilution will be transferred to the ELISAPlate. For Serial Dilution Volumes, the Transfer Volume is taken out of the sample and added to a second well with the Diluent Volume of the Diluent. It is mixed, then the Transfer Volume is taken out of that well to be added to a third well. This is repeated to make Number Of Dilutions diluted samples. For example, if a 100 ug/ ml sample with a Transfer Volume of 20 Microliters, a Diluent Volume of 60 Microliters and a Number of Dilutions of 3 is used, it will create a DilutionCurve of 25 ug/ ml, 6.25 ug/ ml, and 1.5625 ug/ ml with each dilution having a volume of 60 Microliters. For Serial Dilution Factors, the sample will be diluted by the dilution factor at each transfer step. IMPORTANT: because the dilution curve does not intrinsically include the original sample, if the original standard solution is to be included in the dilution curve, the first diluting factor should be set to 1 or Diluent Volume should be 0 Microliters. During experiment, an 5% extra volume than specified is going to be prepared in order to offset pipetting errors. Therefore, the total volume for each well specified in this option should be equal or greater than the volume needed for the experiment. Because the dilution of each assay (well) is prepared independently, the NumberOfReplications should not be considered when determining the Standard Volume. The plate used for sample dilutions will be discarded after the experiment. Note: if spike or antibodies are to be mixed with samples, their volumes are counted towards diluent volume.</div>
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<div class="Figure" id="1464239054"><a name="1464239054"></a><strong>Figure 3.3: </strong>Use the SerialDilutionCuve option to create a collection of serial dilutions that will be performed on standards.</div>
<div class="Bullet" id="1450669605"><a name="1450669605"></a>Default Value: Automatic</div>
<div class="Bullet" id="1022206844"><a name="1022206844"></a>Default Calculation: Automatically set to Null if the StandardDilutionCurve is specified. In all other cases it is automatically set to a Standard Volume of 100 Microliters and a Constant Dilution Factor of 0.5. The Number Of Dilutions is automatically set to 6.</div>
<div class="Bullet" id="361251108"><a name="361251108"></a>Pattern Description: Serial Dilution Factor or Serial Dilution Volumes or Null.</div>
<div class="Bullet" id="611102087"><a name="611102087"></a>Programmatic Pattern: (({GreaterEqualP[0*Microliter], {RangeP[0, 1], GreaterP[0, 1]} | {RangeP[0, 1]..}} | {RangeP[0*Microliter, 1000*Microliter], RangeP[40*Microliter, 1000*Microliter], GreaterP[0, 1]}) | Automatic) | Null</div>
<div class="Bullet" id="1525976255"><a name="1525976255"></a>Index Matches to: Standard</div>
<h4 class="Subsubsubsection"><a name="1833205311"></a><strong>StandardDilutionCurve</strong></h4>
<div class="Text"><a name="733778468"></a>The collection of dilutions that will be performed on each sample. For Fixed Dilution Volume Dilution Curves, the Standard Amount is the volume of the sample that will be mixed the Diluent Volume of the Diluent to create a desired concentration. The Standard Volume is the TOTAL volume of the sample that will created after being diluted by the Dilution Factor. For example, a 1 ug/ ml sample with a dilution factor of 0.7 will be diluted to a concentration 0.7 ug/ ml. IMPORTANT: because the dilution curve does not intrinsically include the original sample, if the original standard solution is to be included in the dilution curve, the first diluting factor should be set to 1 or Diluent Volume must be 0 Microliter. During experiment, an 5% extra volume than specified is going to be prepared in order to offset pipetting errors. Therefore, the total volume for each well specified in this option should be equal or greater than the volume needed for the experiment. Because the dilution of each assay (well) is prepared independently, the NumberOfReplications should not be considered when determining the Standard Volume. The plate used for sample dilutions will be discarded after the experiment. Note: if spike or antibodies are to be mixed with samples, their volumes are counted towards diluent volume.</div>
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<div class="Figure" id="1434091920"><a name="1434091920"></a><strong>Figure 3.4: </strong>Use the DilutionCuve option to create a collection of dilutions that will be performed on standards.</div>
<div class="Bullet" id="1573929159"><a name="1573929159"></a>Default Value: Automatic</div>
<div class="Bullet" id="323811567"><a name="323811567"></a>Default Calculation: Automatically set to Null if the SerialDilutionCurve is specified. If SerialDilutionCurve is set to Null, then automatically set Standard Assay Volume to 100ul, dilution factor to 0.5, 0,25, 0.125, 0.0625, 0.03125, 0.015625.</div>
<div class="Bullet" id="577445071"><a name="577445071"></a>Pattern Description: Fixed Dilution Factor or Fixed Dilution Volume or Null.</div>
<div class="Bullet" id="640707504"><a name="640707504"></a>Programmatic Pattern: (({{RangeP[0*Microliter, 1000*Microliter], RangeP[0*Microliter, 1000*Microliter]}..} | {{RangeP[40*Microliter, 1500*Microliter], RangeP[0, 1]}..}) | Automatic) | Null</div>
<div class="Bullet" id="888013670"><a name="888013670"></a>Index Matches to: Standard</div>
<h4 class="Subsubsubsection"><a name="1981888687"></a><strong>StandardDiluent</strong></h4>
<div class="Text"><a name="2131797439"></a>The buffer used to perform multiple dilutions on Standards.</div>
<div class="Bullet" id="502390725"><a name="502390725"></a>Default Value: Automatic</div>
<div class="Bullet" id="1922698718"><a name="1922698718"></a>Default Calculation: If StandardDilutionCurve and StandardSerialDilutionCurve are not both Null, in the case when Method is set to DirectELISA and IndirectELISA, the option is automatically set to Model[Sample, StockSolution, "1x Carbonate-Bicarbonate Buffer pH10"]; in all other cases, the option is automatically set to Model[Sample,"ELISA Blocker Blocking Buffer"].</div>
<div class="Bullet" id="1610917017"><a name="1610917017"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="1355966990"><a name="1355966990"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<h4 class="Subsubsubsection"><a name="640738966"></a><strong>StandardStorageCondition</strong></h4>
<div class="Text"><a name="1416795504"></a>The condition under which the unused portion of Standard stock sample should be stored.</div>
<div class="Bullet" id="783357792"><a name="783357792"></a>Default Value: Automatic</div>
<div class="Bullet" id="1819654034"><a name="1819654034"></a>Default Calculation: If Standard is Specified, the option is automatically set to Freezer.</div>
<div class="Bullet" id="610732511"><a name="610732511"></a>Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, BacteriaIncubation, MammalianIncubation, TissueCultureCellsIncubation, MicrobialCellsIncubation, MicrobialCellsShakingIncubation, YeastCellsIncubation, YeastCellsShakingIncubation, ViralIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.</div>
<div class="Bullet" id="1756963364"><a name="1756963364"></a>Programmatic Pattern: ((SampleStorageTypeP | Disposal) | Automatic) | Null</div>
<div class="Bullet" id="1383112498"><a name="1383112498"></a>Index Matches to: Standard</div>
<h4 class="Subsubsubsection"><a name="1608758758"></a><strong>StandardCoatingAntibody</strong></h4>
<div class="Text"><a name="1589329976"></a>The sample containing the antibody that is used for coating in FastELISA.</div>
<div class="Bullet" id="1467305246"><a name="1467305246"></a>Default Value: Automatic</div>
<div class="Bullet" id="1444551021"><a name="1444551021"></a>Default Calculation: If Method is FastELISA and Standard is not Null, automatically set to an antibody against a tag which is conjugated to a the StandardCaptureAntibody.</div>
<div class="Bullet" id="1266380612"><a name="1266380612"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="348520467"><a name="348520467"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<div class="Bullet" id="1395583500"><a name="1395583500"></a>Index Matches to: Standard</div>
<h4 class="Subsubsubsection"><a name="755491966"></a><strong>StandardCoatingAntibodyDilutionFactor</strong></h4>
<div class="Text"><a name="1630151498"></a>The dilution ratio of StandardCoatingAntibody. StandardCoatingAntibody is diluted with CoatingAntibodyDiluent. Either StandardCoatingAntibodyDilutionFactor or StandardCoatingAntibodyVolume should be provided but not both.</div>
<div class="Bullet" id="359858460"><a name="359858460"></a>Default Value: Automatic</div>
<div class="Bullet" id="827187268"><a name="827187268"></a>Default Calculation: If Method is FastELISA and Standard is not Null, automatically set to 0.001 (1:1,000)</div>
<div class="Bullet" id="1874766245"><a name="1874766245"></a>Pattern Description: Greater than or equal to 0 and less than or equal to 1 or Null.</div>
<div class="Bullet" id="1316210931"><a name="1316210931"></a>Programmatic Pattern: (RangeP[0, 1] | Automatic) | Null</div>
<div class="Bullet" id="328069570"><a name="328069570"></a>Index Matches to: Standard</div>
<h4 class="Subsubsubsection"><a name="1274741141"></a><strong>StandardCoatingAntibodyVolume</strong></h4>
<div class="Text"><a name="1277494315"></a>The volume of undiluted StandardCoatingAntibody added into the corresponding well of the assay plate. StandardCoatingAntibody is diluted with CoatingAntibodyDiluent. StandardCoatingAntibodyVolume is used as an alternative to StandardCoatingAntibodyDilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.</div>
<div class="Bullet" id="305449499"><a name="305449499"></a>Default Value: Null</div>
<div class="Bullet" id="1203813363"><a name="1203813363"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="1047753554"><a name="1047753554"></a>Programmatic Pattern: RangeP[0*Microliter, 200*Microliter] | Null</div>
<div class="Bullet" id="228076678"><a name="228076678"></a>Index Matches to: Standard</div>
<h4 class="Subsubsubsection"><a name="26417251"></a><strong>StandardCaptureAntibody</strong></h4>
<div class="Text"><a name="1613106275"></a>The sample containing the antibody that is used to pull down the antigen from sample solution to the plate in DirectSandwichELISA, IndirectSandwichELISA, and FastELISA.</div>
<div class="Bullet" id="1643604197"><a name="1643604197"></a>Default Value: Automatic</div>
<div class="Bullet" id="973587618"><a name="973587618"></a>Default Calculation: Automatically set to Null is Standard is Null. If Method is FastELISA, automatically set to an antibody containing an affinity tag and against the TargetAntigen. If Method is DirectSandwichELISA or IndirectSandwichELISA, automatically set to an un-tagged antibody against TargetAntigen.</div>
<div class="Bullet" id="1409189233"><a name="1409189233"></a>Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.</div>
<div class="Bullet" id="1793779915"><a name="1793779915"></a>Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null</div>
<div class="Bullet" id="1683594819"><a name="1683594819"></a>Index Matches to: Standard</div>
<h4 class="Subsubsubsection"><a name="933590061"></a><strong>StandardCaptureAntibodyDilutionFactor</strong></h4>
<div class="Text"><a name="1332630245"></a>The dilution ratio of StandardCaptureAntibody. For DirectSandwichELISA and IndirectSandwichELISA, StandardCaptureAntibody is diluted with CaptureAntibodyDiluent. For FastELISA, StandardCaptureAntibody is diluted in the corresponding standard. Either StandardCaptureAntibodyDilutionFactor or StandardCaptureAntibodyVolume should be provided but not both.</div>
<div class="Bullet" id="1409573152"><a name="1409573152"></a>Default Value: Automatic</div>
<div class="Bullet" id="1803855607"><a name="1803855607"></a>Default Calculation: When Standard is not Null, if Method is DirectSandwichELISA or IndirectSandwichELISA, automatically set to 0.001 (1:1,000). If Method is FastELISA, automatically set to 0.01 (1:100).</div>
<div class="Bullet" id="1374542150"><a name="1374542150"></a>Pattern Description: Greater than or equal to 0 and less than or equal to 1 or Null.</div>
<div class="Bullet" id="1448324271"><a name="1448324271"></a>Programmatic Pattern: (RangeP[0, 1] | Automatic) | Null</div>
<div class="Bullet" id="258883952"><a name="258883952"></a>Index Matches to: Standard</div>
<h4 class="Subsubsubsection"><a name="260712442"></a><strong>StandardCaptureAntibodyVolume</strong></h4>
<div class="Text"><a name="927372814"></a>The volume of undiluted CaptureAntibody added into the corresponding well of the assay plate. StandardCaptureAntibodyVolume is used as an alternative to StandardCaptureAntibodyDilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.</div>
<div class="Bullet" id="2066058619"><a name="2066058619"></a>Default Value: Null</div>
<div class="Bullet" id="1584121190"><a name="1584121190"></a>Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 microliters or Null.</div>
<div class="Bullet" id="1970068471"><a name="1970068471"></a>Programmatic Pattern: RangeP[0*Microliter, 200*Microliter] | Null</div>
<div class="Bullet" id="1037922651"><a name="1037922651"></a>Index Matches to: Standard</div>
<h4 class="Subsubsubsection"><a name="356330776"></a><strong>StandardReferenceAntigen</strong></h4>
<div class="Text"><a name="1666944396"></a>The sample containing the antigen that is used in DirectCompetitiveELISA or IndirectCompetitiveELISA. The StandardReferenceAntigen competes with sample antigen for the binding of the StandardPrimaryAntibody. Reference antigen is sometimes also referred to as inhibitor antigen.</div>
<div class="Bullet" id="262804810"><a name="262804810"></a>Default Value: Automatic</div>